PMID: 3756219Oct 1, 1986Paper

Importance of endogenous substrates for cultured adult rat cardiac myocytes

Biochimica Et Biophysica Acta
H M PiperI Probst

Abstract

In Ca-tolerant adult cardiomyocytes the contribution of endogenous substrates (glycogen, tri- and diacylglycerol) to oxidative substrate metabolism was investigated. After 4 h in culture medium (M 199 plus 4% fetal calf serum) the cellular triacylglycerol content is 3.6-fold higher than in fresh myocardium and reflects the free fatty acid composition of the medium. When triacylglycerol is degraded, all long-chain fatty acids are hydrolysed at equal rates. In these quiescent cells, the activity of pyruvate dehydrogenase is low (10% of full activity, in Tyrode solution with 5 mM glucose). Up to 30% of full pyruvate dehydrogenase activity, the contribution of non-lipid substrates (glycogen, glucose, lactate and pyruvate) to oxidative energy production is correlated to pyruvate dehydrogenase activity. At 5 mM medium concentration, glucose, lactate and pyruvate share in energy production the proportions of 15, 36 and 50%, whereas endogenous lipolysis accounts for 78, 61 and 46%. It is concluded that these quiescent cardiomyocytes represent cardiac metabolism in a basal state in which the preference for fatty acids, especially from endogenous lipids, is very pronounced. The utilization of endogenous substrates therefore has to be con...Continue Reading

References

Nov 1, 1979·Basic Research in Cardiology·H KahlesP G Spieckermann
Feb 1, 1979·Circulation Research·K Kobayashi, J R Neely
Sep 1, 1979·Journal of Molecular and Cellular Cardiology·F J PearceG F Tutwiler
Dec 1, 1985·Journal of Molecular and Cellular Cardiology·P M LatipääI E Hassinen
Oct 1, 1966·Metabolism: Clinical and Experimental·J Scheuer, N Brachfeld
Mar 1, 1984·Journal of Molecular and Cellular Cardiology·J F HütterP G Spieckermann
May 1, 1984·Basic Research in Cardiology·J R Williamson, K Kobayashi
Aug 1, 1983·Biochimica Et Biophysica Acta·G J Van der VusseR S Reneman
Jun 1, 1982·Journal of Molecular and Cellular Cardiology·D H Hunneman, C Schweickhardt
Jul 1, 1982·Journal of Molecular and Cellular Cardiology·H M PiperP G Spieckermann

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Citations

Feb 11, 2003·Cell Structure and Function·Maria de Nazaré Correia SoeiroMaria de Nazareth Leal Meirelles

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