Importance of ERK1/2 in upregulation of endothelin type B receptors in cerebral arteries

British Journal of Pharmacology
Marie HenrikssonLars Edvinsson

Abstract

This study examines the importance of mitogen-activated protein kinases (MAPKs) in upregulation of endothelin type B (ETB) receptors. Rat middle cerebral arteries (MCAs) were incubated for 24 h with or without kinase inhibitors. Vessel segments were mounted in myographs and the contractile responses to endothelin-1 (ET-1; ETA and ETB receptor agonist) and sarafotoxin 6c (S6c; ETB receptor agonist) were studied. We used real-time PCR to measure the receptor mRNA levels. An ELISA assay showed the activation of ERK1/2 kinases after 3 h. Immunohistochemistry revealed the presence of ETB receptors on the vessels. After organ culture, S6c induced vasoconstriction. Incubation with the MEK/ERK inhibitors U0126 and SB386023 diminished the contractile response to S6c. The p38 MAPK inhibitor SB239063 did not affect the S6c-induced contraction. The ET-1-induced vasoconstriction was increased after incubation with SB386023 or SB239063, while unaffected by U0126. The ETB receptor mRNA levels were diminished by SB386023 and U0126. The ETA receptor mRNA levels were unaffected. The levels of activated ERK1/2 kinases were significantly higher after 3 h of organ culture as compared to fresh vessels. The level of ETB receptor protein on the smooth...Continue Reading

References

Dec 20, 1995·European Journal of Pharmacology·N J LodgeS Moreland
Apr 1, 1997·Current Eye Research·F P Diecke, A Beyer-Mears
Apr 30, 1998·Advances in Cancer Research·T S LewisN G Ahn
Jul 11, 1998·The Journal of Biological Chemistry·M F FavataJ M Trzaskos
Mar 19, 1999·Molecular and Cellular Biology·H J Schaeffer, M J Weber
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·A AlessandriniJ V Bonventre
Feb 5, 2000·Stroke; a Journal of Cerebral Circulation·S M DavisJ Norris
Aug 16, 2001·Proceedings of the National Academy of Sciences of the United States of America·S NamuraA Alessandrini
Sep 7, 2002·Stroke; a Journal of Cerebral Circulation·Emelie StenmanLars Edvinsson
Sep 10, 2002·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Shohei MatsumotoTadeusz Wieloch
Dec 4, 2002·Acta Neurologica Scandinavica·F LennmyrA Terént
May 17, 2003·Stroke; a Journal of Cerebral Circulation·Marie HenrikssonLars Edvinsson
Nov 11, 2003·Trends in Pharmacological Sciences·ChengFeng Yang, Marcelo G Kazanietz

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Citations

Nov 9, 2006·Experimental Brain Research·Marie HenrikssonLars Edvinsson
Nov 23, 2006·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·P Vikman, L Edvinsson
Jun 3, 2008·Canadian Journal of Physiology and Pharmacology·Fernando S CarneiroR Clinton Webb
Jun 16, 2010·Pharmacology & Therapeutics·Cang-Bao XuLars Edvinsson
Nov 23, 2007·European Journal of Pharmacology·David NilssonMalin Malmsjö
Oct 30, 2007·European Journal of Pharmacology·David NilssonMalin Malmsjö
Sep 12, 2015·Translational Stroke Research·Yu HasegawaShokei Kim-Mitsuyama
Jan 26, 2006·Basic & Clinical Pharmacology & Toxicology·Yong-Xiao CaoLars Edvinsson
May 3, 2006·European Journal of Pharmacology·Guogang LuoCang-Bao Xu
Jun 15, 2015·International Immunopharmacology·Vengadeshprabhu KaruppagounderKenichi Watanabe
Oct 28, 2005·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Saema A S BegLars I H Edvinsson
May 12, 2011·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Lars I H Edvinsson, Gro Klitgaard Povlsen
Apr 28, 2005·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Kazuki EndoKatsuo Kamata

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