Importance of hydrophobic region in amphiphilic structures of alpha-helical peptides for their gene transfer-ability into cells

Biochemical and Biophysical Research Communications
N OhmoriH Aoyagi

Abstract

It has been showned that cationic alpha-helical peptides can be useful as nucleic acid-carrier molecules for gene transfer into cells. In order to investigate the significancemake sure of importance of the hydrophobic region in amphiphilic peptides in relation to their transfection ability, we have employed five kinds of peptides with a systematically varied hydrophobic-hydrophilic balance in the amphiphilic structures, and have evaluated the relationship between the structure and the gene transfer ability of the peptides into COS-7 cells. The peptides with a large hydrophobic region took alpha-helical structures, formed large aggregates and showed high transfection efficiency. Their high efficiency can be explained on the basis of their ability to form stable aggregates which can be internalized by endocytosis and remain resistant to digestion in lysosomal vesicles. Furthermore, it was suggested that the hydrophobic region of peptides plays an important role in the disruption of the endosomal membrane, which ca prevent the degradation of DNA in lysosomal vesicles. When peptides do not have so strong membrane-disruptive activity, but form aggregates which can be incorporated by endocytosis, the transfection efficiency can be re...Continue Reading

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Citations

Aug 8, 2002·Clinical and Experimental Pharmacology & Physiology·Joseph I Kourie, Christine L Henry
Oct 26, 1999·The Journal of Peptide Research : Official Journal of the American Peptide Society·T NiidomeH Aoyagi
Dec 19, 2008·Journal of Peptide Science : an Official Publication of the European Peptide Society·Shinichi KuriyamaTakuro Niidoime
Jun 10, 2015·Journal of Nanobiotechnology·Baoling ChenP Chen
Mar 21, 2006·Biochimica Et Biophysica Acta·Sílvia PujalsErnest Giralt
Nov 17, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Shubiao ZhangZongshi Li
Apr 1, 2004·Biopolymers·Jimena Fernández-CarneadoErnest Giralt
Feb 25, 2010·Journal of Biomaterials Science. Polymer Edition·Takami AkagiMitsuru Akashi
May 20, 2006·Journal of Peptide Science : an Official Publication of the European Peptide Society·Shinichi KuriyamaTakuro Niidome
Jan 29, 2021·International Journal of Pharmaceutics·Emma M McErleanHelen O McCarthy
Jan 25, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Hiromichi NakaharaOsamu Shibata
Dec 20, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·Anas El-Aneed
Nov 29, 2008·International Journal of Pharmaceutics·Geetha N GoparajuPardeep K Gupta
Oct 15, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society· RajpalMunia Ganguli

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