Importance of hydroxyls at positions 3, 4, and 6 for binding to the "galactose" site of beta-galactosidase (Escherichia coli)

Archives of Biochemistry and Biophysics
R E Huber, M T Gaunt

Abstract

Values of kinetic inhibition constants representing dissociation constants of inhibitors interacting with the free form of beta-galactosidase ('galactose" site) were determined for a large number of monosaccharides and alcohols. The studies showed that when hydroxyl groups were present at positions 3, 4, and 6 and were in the same orientation as in galactose, binding to the 'galactose" site was good. Alterations in the position of or a lack of a hydroxyl at any of these three positions decreased binding dramatically. The overall binding specificity thus depends to a large extent on these three positions. Positions 3 and 4 were critical. A misorientation at either position eliminated most of the binding. Position 6 was a little more independent. The absence of a hydroxyl at that position did not totally eliminate the binding. The position 2 hydroxyl did not seem to be important for binding as its absence or orientation had only a small effect on the binding capacity. Studies regarding position 5 were inconclusive but since a minimum of four hydroxyl groups seemed important for binding and since positions 3, 4, and 6 are important the 5 position may also be of significance. The work also showed that p-nitrophenyl-beta-D-glucopyra...Continue Reading

References

Jun 29, 1978·Biochemical and Biophysical Research Communications·H Woulfe-Flanagan, R E Huber
Sep 1, 1975·Canadian Journal of Biochemistry·R E HuberG Kurz
Apr 10, 1969·Biochemical and Biophysical Research Communications·Y C Lee
Sep 21, 1973·European Journal of Biochemistry·G Van der GroenC K De Bruyne
Mar 1, 1969·Archives of Biochemistry and Biophysics·S Shifrin, G Hunn
Jun 11, 1971·European Journal of Biochemistry·J P TenuJ Yon
Feb 1, 1981·Canadian Journal of Biochemistry·R E HuberC L Turner
Jun 1, 1982·Canadian Journal of Biochemistry·R E Huber, M T Gaunt
Aug 1, 1961·The Biochemical Journal·G N WILKINSON

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Citations

Mar 1, 1994·The International Journal of Biochemistry·R E HuberS K Khare
Nov 21, 1997·Biochimica Et Biophysica Acta·S LeparouxB Colas
Oct 6, 2010·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Megan L DugdaleReuben E Huber
Nov 26, 2010·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Megan L DugdaleReuben E Huber
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·J H ZhangW P Stemmer
Sep 27, 2012·Protein Science : a Publication of the Protein Society·Douglas H JuersReuben E Huber
Nov 28, 2006·Biochemical and Biophysical Research Communications·Gopinath SutendraReuben E Huber
Apr 25, 2017·Acta Crystallographica. Section E, Crystallographic Communications·Bruno Leonardo SilvaNivaldo Lúcio Speziali
May 28, 2009·Protein Science : a Publication of the Protein Society·Douglas H JuersReuben E Huber
Jun 15, 2010·Chembiochem : a European Journal of Chemical Biology·Rémi CaraballoOlof Ramström
Apr 1, 1986·Archives of Biochemistry and Biophysics·R E Huber, K L Hurlburt
Sep 16, 1985·Biochemical and Biophysical Research Communications·M RingR E Huber
Oct 1, 1984·Archives of Biochemistry and Biophysics·R E HuberK L Hurlburt
May 11, 2018·The Journal of Physical Chemistry. B·Matthias J Mickert, Hans H Gorris

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