Importance of integrity of cell-cell junctions for the mechanics of confluent MDCK II cells

Scientific Reports
Bastian Rouven Brückner, Andreas Janshoff

Abstract

Intercellular junctions are important mechanical couplers between cells in epithelial layers providing adhesion and intercellular communication. Regulation of the junctions occurs in cellular processes such as layer formation, epithelial-to-mesenchymal transition, embryogenesis, and cancer progression. Many studies addressed the role of force generation in cells for establishing lateral cell-cell junctions and the role of cellular force transmission in tissue formation and maintenance. Our atomic force microscopy- (AFM) based study shed light on the role of both, tight junctions and adherens junctions for the mechanical properties of individual epithelial cells that are part of a confluent monolayer. We found that tight junctions are important for the establishment of a functional barrier-forming layer but impairing them does not reduce the mechanical integrity of cells. Depletion of ZO-1 results in a weak increase in cortical tension. An opposite effect was observed for disruption of E-cadherin-mediated adherens junctions using DTT. Opening of adherens junctions leads to substantial alterations of cellular mechanics such as reduced overall stiffness, but these changes turned out to be reversible after re-establishing disulfide...Continue Reading

References

Dec 1, 1990·Mechanisms of Development·M OzawaR Kemler
Aug 18, 1989·Science·E Rodriguez-Boulan, W J Nelson
Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·D L RimmJ S Morrow
Feb 9, 1996·Cell·N M Kumar, N B Gilula
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·R J Pelham, Y l Wang
Dec 10, 1999·Biophysical Journal·J Dai, M P Sheetz
Sep 26, 2003·Biophysical Journal·Alina HateganDennis E Discher
Dec 2, 2004·Cell Motility and the Cytoskeleton·Tony YeungPaul A Janmey
May 9, 2006·Proceedings of the National Academy of Sciences of the United States of America·F Brochard-WyartP Nassoy
Mar 14, 2007·The Journal of Cell Biology·Junichi IkenouchiShoichiro Tsukita
Nov 7, 2008·Journal of Cell Science·Maria S Balda, Karl Matter
Jul 17, 2009·Molecular Biology of the Cell·Christina M Van ItallieJames M Anderson
Nov 13, 2009·Current Biology : CB·Shang-You TeePaul A Janmey
Feb 18, 2010·Biophysical Journal·Benoit LadouxRené-Marc Mège
May 11, 2010·Nature Cell Biology·Shigenobu YonemuraMai Shibata
May 14, 2010·Proceedings of the National Academy of Sciences of the United States of America·Zhijun LiuChristopher S Chen
Jun 24, 2010·Nature Reviews. Molecular Cell Biology·Tony J C Harris, Ulrich Tepass
Aug 19, 2010·The EMBO Journal·Ekaterina Papusheva, Carl-Philipp Heisenberg
Oct 13, 2010·Annual Review of Physiology·Le ShenJerrold R Turner
Jun 15, 2011·Trends in Cell Biology·Daniel J Müller, Yves F Dufrêne
Aug 3, 2011·Current Opinion in Cell Biology·Shigenobu Yonemura
May 5, 2012·Trends in Cell Biology·Julia BraschLawrence Shapiro
Nov 6, 2012·Trends in Cell Biology·Alba Diz-MuñozOrion D Weiner
May 30, 2013·Proceedings of the National Academy of Sciences of the United States of America·David W DumbauldAndrés J García
May 16, 2014·Nature Reviews. Molecular Cell Biology·Masatoshi Takeichi
Mar 31, 2015·Nature Cell Biology·Elsa BazellièresXavier Trepat
Jul 16, 2015·Mucosal Immunology·G LeoniA Nusrat
Jul 18, 2015·Current Opinion in Cell Biology·Andrew G Clark, Danijela Matic Vignjevic
Oct 6, 2015·Scientific Reports·Bastian Rouven BrücknerAndreas Janshoff
Jun 30, 2016·Nature Reviews. Molecular Cell Biology·Ceniz ZihniMaria S Balda
Jan 18, 2017·Cold Spring Harbor Perspectives in Biology·Mechthild HatzfeldThomas M Magin
Apr 5, 2017·Experimental Cell Research·Hannah K CampbellKris A DeMali
May 13, 2017·Molecular Biology of the Cell·Joshua A BroussardHoracio D Espinosa
Oct 19, 2017·Nature Communications·Alexander X Cartagena-RiveraRichard S Chadwick
Nov 14, 2017·Biophysical Journal·Susanne KarschAndreas Janshoff

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Citations

May 3, 2019·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Andrea N RobinsonMichael M Gottesman
Oct 6, 2019·Biophysics Reviews·Sandra Citi
Jan 9, 2020·Cellular and Molecular Life Sciences : CMLS·Susanne KarschAndreas Janshoff
Jul 28, 2020·Frontiers in Cell and Developmental Biology·Valentina DamianoManuela Santarosa
Nov 3, 2020·Alternatives to Laboratory Animals : ATLA·Adrián García-SalvadorFelipe Goñi-de-Cerio
Dec 15, 2020·Journal of the Mechanical Behavior of Biomedical Materials·Faezeh EskandariKaveh Laksari
Dec 10, 2020·International Journal of Molecular Sciences·Claudia Giuseppina FrestaClaudio Bucolo
Mar 27, 2021·Nature Communications·K Venkatesan IyerFrank Jülicher
Jul 8, 2020·ACS Applied Materials & Interfaces·Hyejeong KimMarco Tarantola

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Methods Mentioned

BETA
AFM
atomic force microscopy
Fluorescence
fluorescence microscopy
transfection
fluorescence imaging

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