Importance of osteoprotegrin and receptor activator of nuclear factor κB in breast cancer response to hepatocyte growth factor and the bone microenvironment in vitro

International Journal of Oncology
Sioned OwenWen G Jiang

Abstract

Osteoprotegrin (OPG), receptor activator of nuclear factor κB (RANK) and RANK ligand (RANKL) are signal transducers which have pleiotropic actions. Each tumour necrosis factor receptor superfamily member has unique structural attributes which directly couples them to signalling pathways involved in cell proliferation, differentiation and survival. Previous studies have clinically linked OPG, RANK and RANKL to increasing tumour burden, metastatic bone involvement and estrogen status. This study aimed to establish the potential implications of targeting endogenously produced OPG and RANK in the osteotropic breast cancer cell line MDA-MB‑231 in vitro. Subsequently this study also aimed to explore the potential links between these molecules with regards to hepatocyte growth factor (HGF) signalling and extracted bone proteins (BME). OPG and RANK expression was successfully suppressed using hammerhead ribozyme technology. Subsequently effects were explored in MDA-MB‑231 cell proliferation, matrix adhesion, migration and invasion in vitro function assays. Reduced OPG expression resulted in increased breast cancer cell migration and invasion. These increases, particularly invasion, appeared to however be reduced under the influence of ...Continue Reading

References

Sep 30, 1992·International Journal of Cancer. Journal International Du Cancer·C R ParishD R Coombe
Jan 1, 1990·Cancer Investigation·E M RosenI D Goldberg
Aug 16, 1984·Biochemical and Biophysical Research Communications·T NakamuraA Ichihara
May 1, 1984·Journal of Cellular Physiology·W E RussellN L Bucher
Oct 29, 1998·Biochemical and Biophysical Research Communications·Y HakedaM Kumegawa
Jun 26, 2003·Nucleic Acids Research·Michael Zuker
Apr 16, 2004·The New England Journal of Medicine·G David Roodman
Dec 21, 2004·Critical Reviews in Oncology/hematology·Wen G JiangToshikazu Nakamura
Oct 26, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Robert E Coleman
Apr 5, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Diptiman ChandaSelvarangan Ponnazhagan
Apr 9, 2008·Archives of Biochemistry and Biophysics·Brendan F Boyce, Lianping Xing
Aug 2, 2008·The Lancet Oncology·Isaiah J Fidler, George Poste
May 20, 2011·Nature Reviews. Cancer·Katherine N WeilbaecherLaurie K McCauley
Mar 1, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kanwal P RaghavAna M Gonzalez-Angulo
Nov 26, 2013·Clinical & Experimental Metastasis·Michelle L BlakeWilliam C Dougall
Jul 1, 2014·Cancer Medicine·Michael WeichhausLinda Connelly

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Methods Mentioned

BETA
PCR
protein assay
motility assay
transfection

Software Mentioned

Sigma plot

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