Importance of platelets and fibrinogen in neutrophil-endothelial cell interactions in septic shock

Critical Care Medicine
Linda A KirschenbaumMark E Astiz

Abstract

To examine the role of platelets, fibrin, and adhesion molecules in mediating neutrophil-endothelial cell interactions in septic shock. Controlled experiments using phase contrast microscopy to examine neutrophil, platelet, and endothelial cell interactions in flowing cell suspensions under simulated physiologic conditions. University research laboratory. Adult patients with septic shock and normal volunteers. Microslides were coated with human umbilical vein endothelial cells. Neutrophils were removed from control subjects and patients in septic shock and were perfused over endothelial cells at rates representing a range of physiologic shear stresses. In an attempt to examine the effects of fibrin deposition on neutrophil-endothelial cell interactions, neutrophils, with and without platelets, were suspended in plasma and serum was removed from patients in septic shock. In addition, blocking monoclonal antibodies against the platelet receptor P-selectin and neutrophil receptor CD11b/CD18, and a platelet glycoprotein IIb/IIIa inhibitor, were incubated with cells suspended in plasma. Phase contrast video microscopy was used to count the number of neutrophils/mm adherent to endothelial cells during cessation of flow. Neutrophil ro...Continue Reading

References

May 1, 1991·Chest·M E AstizM H Weil
Jun 1, 1990·American Journal of Respiratory Cell and Molecular Biology·S K YoungP M Henson
Nov 1, 1987·Microvascular Research·S D House, H H Lipowsky
Feb 1, 1995·Critical Care Medicine·M E AstizE C Rackow
Nov 1, 1994·The Journal of Clinical Investigation·C LamW Sibbald
Jan 1, 1996·American Journal of Respiratory Cell and Molecular Biology·K J LeavellT J Gross
Nov 1, 1995·European Journal of Clinical Investigation·M GawazK Werdan
Mar 1, 1996·The Journal of Surgical Research·J M LiA D Michelson
Jan 1, 1997·American Journal of Respiratory and Critical Care Medicine·P C YodiceE C Rackow
Aug 14, 1999·Critical Care Medicine·C Ince, M Sinaasappel
May 12, 2000·American Journal of Respiratory and Critical Care Medicine·L A KirschenbaumE C Rackow
Jul 17, 2001·American Journal of Physiology. Heart and Circulatory Physiology·J KaurP Kubes
Nov 14, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·J W SalterD N Granger
Jul 2, 2002·American Journal of Respiratory and Critical Care Medicine·Daniel De BackerJean-Louis Vincent

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Citations

Jan 20, 2006·Critical Care : the Official Journal of the Critical Care Forum·Roland S CronerErnst Klar
Oct 25, 2012·Intensive Care Medicine·Roberto Alberto De BlasiRoberto Arcioni
Aug 30, 2014·Critical Care Nursing Clinics of North America·Shannan K HamlinSandra K Hanneman
Mar 3, 2011·Chest·Jason N KatzRichard C Becker
Apr 4, 2014·Thrombosis and Haemostasis·Tom van der Poll, Heiko Herwald
Sep 19, 2006·The Journal of Pathology·D Cruz-TopeteF J Castellino
Dec 19, 2009·Journal of Veterinary Emergency and Critical Care·Deborah C SilversteinKenneth J Drobatz
May 6, 2016·Frontiers in Cellular Neuroscience·M Akhtar AnwarAli H Eid
Sep 4, 2007·Atherosclerosis·Gaetano VaudoElmo Mannarino
May 1, 2007·Current Opinion in Critical Care·Gustavo Luiz BücheleDaniel De Backer
Jan 24, 2008·Blood Purification·P ToftK E Olsen
Apr 25, 2009·Current Opinion in Anaesthesiology·Daniel De BackerRaphaël Favory
Feb 25, 2009·Critical Care Medicine·Michael Piagnerelli
Mar 25, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·G Thomas Brown, Thomas M McIntyre
Feb 22, 2017·The International Journal of Artificial Organs·Tanja EichhornViktoria Weber
Sep 25, 2009·Anaesthesia and Intensive Care·S NanasC Roussos
Nov 29, 2017·Immunological Investigations·Fanny PoujolFabienne Venet
Jun 22, 2021·Frontiers in Immunology·Xiang-Zhi FangYou Shang

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