Importance of Preferential Flow and Soil Management in Vertical Transport of a Biocontrol Strain of Pseudomonas fluorescens in Structured Field Soil.

Applied and Environmental Microbiology
A NatschGeneviève Défago

Abstract

The large-scale release of wild-type or genetically modified bacteria into the environment for control of plant diseases or for bioremediation entails the potential risk of groundwater contamination by these microorganisms. For a model study on patterns of vertical transport of bacteria under field conditions, the biocontrol strain Pseudomonas fluorescens CHA0, marked with a spontaneous resistance to rifampin (CHA0-Rif), was applied to a grass-clover ley plot (rotation grassland) and a wheat plot. Immediately after bacterial application, heavy precipitation was simulated by sprinkling, over a period of 8 h, 40 mm of water containing the mobile tracer potassium bromide and the dye Brilliant Blue FCF to identify channels of preferential flow. One day later, a 150-cm-deep soil trench was dug and soil profiles were prepared. Soil samples were extracted at different depths of the profiles and analyzed for the number of CHA0-Rif cells and the concentration of bromide and Brilliant Blue FCF. Dye coverage in the soil profiles was estimated by image analysis. CHA0 was present at 10(sup8) CFU/g in the surface soil, and 10(sup6) to 10(sup7) CFU/g of CHA0 was detected along macropores between 10 and 150 cm deep. Similarly, the concentratio...Continue Reading

References

Jan 1, 1991·Biotechnology·D F Berry, C Hagedorn
Oct 1, 1985·Applied and Environmental Microbiology·R M GoldsteinM Alexander
Jan 1, 1991·Applied and Environmental Microbiology·J T GannonM Alexander

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Citations

Jan 16, 2002·Journal of Environmental Quality·M McLeodM Taylor
Oct 24, 2008·Phytopathology·W E SnyderD A Kluepfel
Jun 25, 2009·Journal of Environmental Quality·Scott A Bradford, Eran Segal
Jan 22, 2005·Environmental Microbiology·Rebekka R E ArtzKen Killham
Feb 18, 2015·Microbial Ecology·Julia MollFrancois Buscot
Mar 22, 2014·Environmental Microbiology·Juliana AlmarioYvan Moënne-Loccoz

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