PMID: 938640Jun 8, 1976Paper

Importance of the flux of phosphate across the inner membrane of kidney mitochondria for the activation of glutaminase and the transport of glutamine

Biochimica Et Biophysica Acta
Z Kovacević

Abstract

The effect of mersalyl, an inhibitor of phosphate transport across the inner mitochondrial membrane, was investigated on the uncoupled respiration of pig kidney mitochondria in the presence of glutamine as substrate and on the activity of the phosphate-dependent glutaminase in the intact organelles. In addition, the submitochondrial location of the enzyme was reinvestigated. (1) It was found that mersalyl completely inhibits uncoupled respiration of the mitochondria in the presence of glutamine as substrate, whereas respiration with glutamate was not affected. The same amount of mersalyl which inhibits coupled oxidation of glutamine also inhibits coupled oxidation of glutamate and some other substrates. (2) Mersalyl strongly inhibited the activation of glutaminase in intact mitochondria only in the presence of inhibitiors of electron transport or of an uncoupler. The addition of a detergent prevented or fully released the inhibition. The effect of mersalyl was observed even when the mitochondria were pre-incubated with phosphate or incubated in the phosphate-free medium. If mersalyl and carbonyl cyanide m-chlorophenylhydrazone (CCCP) were added 3 min after pre-incubation with phosphate the same intramitochondrial concentration ...Continue Reading

References

Mar 25, 1975·Biochimica Et Biophysica Acta·T Gulik-Krzywicki
Mar 1, 1975·Archives of Biochemistry and Biophysics·M A Matlib, P J O'Brien
Jan 1, 1974·Annual Review of Biochemistry·S J Singer
Jan 1, 1973·The Biochemical Journal·M CromptonJ B Chappell
Dec 14, 1973·Biochimica Et Biophysica Acta·J Meyer, P M Vignais
Mar 1, 1974·The American Journal of Physiology·T C Welbourne
Jun 1, 1970·The Biochemical Journal·Z KovacevićJ B Chappell
Jan 1, 1967·Advances in Enzyme Regulation·N KatunumaI Tomino
Jul 1, 1968·The Journal of Cell Biology·C Schnaitman, J W Greenawalt
Jul 6, 1966·Biochemical and Biophysical Research Communications·A Fonyo, S P Bessman

❮ Previous
Next ❯

Citations

Jan 1, 1980·The International Journal of Biochemistry·Z KovacevićM Pavlovic
Feb 1, 1983·Journal of Neuroscience Methods·J Butterworth, C M Yates
Oct 2, 2009·Journal of Bone and Mineral Metabolism·Atsushi SuzukiJoseph Caverzasio
Aug 1, 1983·Journal of Neurochemistry·J ButterworthJ Simpson
Nov 15, 1985·Archives of Biochemistry and Biophysics·R A ShapiroN P Curthoys

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Landau-Kleffner Syndrome

Landau Kleffner syndrome (LKS), also called infantile acquired aphasia, acquired epileptic aphasia, or aphasia with convulsive disorder, is a rare childhood neurological syndrome characterized by the sudden or gradual development of aphasia (the inability to understand or express language) and an abnormal electroencephalogram. Discover the latest research on LKS here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.