PMID: 4891041Mar 1, 1969Paper

Importance of the osmolarity of the incubation medium on amino acid incorporation into protein by isolated rat liver mitochondria

The Biochemical Journal
D Haldar, K B Freeman

Abstract

1. Incorporation of [(14)C]leucine into protein by isolated rat liver mitochondria was examined by using incubation media similar to those used by Sandell, Löw & Decken (1967) (medium A) and Roodyn, Reis & Work (1961) (medium B). The incorporation process was found to be almost completely inhibited in medium A. 2. By decreasing the amount of sucrose and omitting tris-hydrochloric acid from medium A, incorporation proceeded at a rate higher than that found in medium B. It was found that the inhibitory action of medium A was due to its high osmolarity. 3. Oxidative phosphorylation and RNA synthesis by the isolated mitochondria proceeded at the same rate in media essentially the same as media A and B. 4. There was a partial inhibitory action of medium A on leucine uptake by the mitochondria and also on the formation of leucyl-transfer-RNA. The major block of inhibition by the hyperosmolarity of medium A seemed to be located at a later step of protein synthesis involving mitochondrial ribosomes. 5. Protein synthesis by Escherichia coli B was only slightly inhibited, if at all, in hyperosmotic media in which protein synthesis by isolated mitochondria was completely stopped.

Citations

Dec 10, 1971·European Journal of Biochemistry·J L Coote, T S Work
Jul 13, 1970·Biochemical and Biophysical Research Communications·D Haldar
Oct 23, 1974·Biochemical and Biophysical Research Communications·R B Wallace, K B Freeman
Mar 1, 1975·Journal of Neurochemistry·S TewariE P Noble
Feb 23, 2020·Journal of Neuromuscular Diseases·Tomoya TaminatoYuji Takahashi
Apr 8, 1971·Life Sciences. Pt. 2: Biochemistry, General and Molecular Biology·J K LynnH Sidransky
Sep 14, 1972·Biochimica Et Biophysica Acta·J Mockel
Mar 1, 1977·Antimicrobial Agents and Chemotherapy·K B Freeman

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