PMID: 8971918Jan 1, 1996Paper

Importance of the proline residue to the functional activity and metabolic stability of the nematode FMRFamide-related peptide, KPNFIRFamide (PF4)

Peptides
T M KubiakJ R Wiest

Abstract

PF4 has previously been shown to have potent inhibitory effects on myoactivity of somatic muscle strips from the nematode. Ascaris suum. This study examined the bioactivity and metabolic stability of position 2- and position 5-modified analogues of PF4. Although the analogues [Leu5]PF4,[Ala2]PF4, [Gly2]PF4, [Ala2,Leu5]PF4, and [Gly2,Leu5]PF4 all had qualitatively similar inhibitory effects on A. suum somatic muscle strips, their effects were quantitatively distinguishable and had the order of potency: PF4 = [Leu5]PF4 > > [Ala2]PF4 = [Ala2,Leu5]PF4 > > [Gly2]PF4 = [Gly2,Leu5]PF4, Leu5 for Ile5 substitutions in PF4 did not alter the activity of this peptide: however, Gly2/Ala2 for Pro2 substitutions reduced, but did not abolish, peptide activity. Peptide stability studies revealed that [Gly2]PF4(2-7) and -(3-7) and [Ala2]PF4(2-7), -(3-7), and -(4-7) fragments were generated following exposure to A. suum somatic muscle strips. However, the parent peptide (PF4) was not metabolized and appeared to be resistant to the sequential cleavages of native aminopeptidases. Observed analogue metabolism appeared to be due to the activity of released aminopeptidases as identical fragments were generated by incubation in medium that had been exp...Continue Reading

References

Apr 18, 1980·Molecular and Cellular Biochemistry·R WalterT Yoshimoto
May 1, 1993·Peptides·C Cowden, A O Stretton
Jan 1, 1993·Critical Reviews in Biochemistry and Molecular Biology·A Yaron, F Naider

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Citations

Mar 14, 2003·International Journal for Parasitology·D P ThompsonT G Geary
Feb 17, 2000·Annals of the New York Academy of Sciences·T G GearyD P Thompson
Apr 28, 2004·International Journal for Parasitology·Angela MousleyAaron G Maule

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