Important role of 5-hydroxytryptamine in glucocorticoid-induced insulin resistance in liver and intra-abdominal adipose tissue of rats

Journal of Diabetes Investigation
Tao LiJihua Fu

Abstract

Both glucocorticoids and 5-hydroxytryptamine (5-HT) have been shown to induce insulin resistance (IR) in hepatocytes and adipocytes. Here, we explore whether there is a correlation between them. Except for the control group, male rats were exposed to dexamethasone treated with or without para-chlorophenylalanine (pCPA), or carbidopa for 20 days. Except for the control group, buffalo rat liver 3A (BRL-3A) cells were exposed to dexamethasone for 24 h, treated with or without pCPA, carbidopa, or clorgiline for 48 h, or exposed to 5-HT treated with or without fluoxetine for 48 h. Whole-body IR was determined by both glucose tolerance test and measurement of fasting blood glucose and insulin, whereas hepatocytes or adipocytes IR was determined by examining either hepatic gluconeogenesis, steatosis and glucose transporter 2 expression or lipolysis. Dexamethasone-induced whole-body IR, liver and intraabdominal adipose IR were accompanied by upregulated expressions of tryptophan hydroxylase-1 and aromatic amino acid decarboxylase with increased 5-HT level in both tissues, which were attenuated significantly by pCPA, inhibiting tryptophan hydroxylase-1, or carbidopa, inhibiting aromatic amino acid decarboxylase. [Correction added on 22 ...Continue Reading

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Citations

Jun 25, 2016·International Journal of Molecular Sciences·Wan-Xia ZhangYou-E Yan
Jul 4, 2020·Endocrinology and Metabolism·Ko Eun ShongHail Kim
Jul 20, 2021·European Journal of Pharmacology·Yu-Xin ZhangJi-Hua Fu

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Methods Mentioned

BETA
immunoprecipitation
reverse transcription PCR
electrophoresis

Software Mentioned

LifeScan
GeneGenius

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