Importin alpha from Arabidopsis thaliana is a nuclear import receptor that recognizes three classes of import signals

Plant Physiology
H M SmithN V Raikhel

Abstract

Protein import into the nucleus is a two-step process. In vitro import systems from vertebrate cell extracts have shown several soluble factors are required. One of these factors is the receptor importin alpha, which binds to nuclear localization signals (NLS) in vitro. We previously cloned an importin alpha homolog from Arabidopsis thaliana (At-IMP alpha) and demonstrated that this protein was not depleted from tobacco (Nicotiana tabacum) protoplasts after permeabilization of the plasma membrane, (Hicks et al., 1996). To determine if At-IMP alpha is functional, we used an in vitro NLS-binding assay. We found that At-IMP alpha is specific, and the receptor is able to recognize three classes of NLS identified in plants. Purified antibodies to At-IMP alpha were used to determine the in vivo location of importin alpha in tobacco protoplasts. Importin alpha is found in the cytoplasm and nucleus, and it is most highly concentrated at the nuclear envelope. The biochemical properties of nuclear importin alpha and localization studies using purified nuclei demonstrate that importin alpha is tightly associated with the plant nucleus. Moreover, these results suggest that a fraction of nuclear importin alpha interacts with the nuclear por...Continue Reading

References

Dec 1, 1991·Trends in Biochemical Sciences·C Dingwall, R A Laskey
Dec 1, 1986·The Journal of Cell Biology·D D NewmeyerD J Forbes
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·J D LoebG R Fink
Apr 1, 1995·Plant Physiology·G R HicksN V Raikhel
Jan 1, 1995·Experimental Cell Research·K Mishra, V K Parnaik
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·G SchlenstedtP A Silver
Jan 31, 1995·Proceedings of the National Academy of Sciences of the United States of America·G R Hicks, N V Raikhel
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·A RaduM S Moore
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·M S Moore, G Blobel
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·P CortesD Baltimore
May 1, 1994·Journal of Cellular Biochemistry·T Boulikas
Feb 1, 1994·The Plant Journal : for Cell and Molecular Biology·M J Varagona, N V Raikhel
Aug 1, 1993·The Plant Cell·G R Hicks, N V Raikhel
Apr 5, 1996·Science·U Nehrbass, G Blobel
Jun 1, 1996·The Journal of Cell Biology·D J Sweet, L Gerace
Jan 1, 1995·Annual Review of Cell and Developmental Biology·G R Hicks, N V Raikhel
Feb 1, 1996·Trends in Biochemical Sciences·M VandrommeA Fernandez

❮ Previous
Next ❯

Citations

Jun 8, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D A JansM H Lam
Oct 21, 2010·Plant Cell Reports·Thomas Merkle
Apr 27, 2000·Progress in Biophysics and Molecular Biology·A S SobolevA A Rosenkranz
Apr 11, 2003·Nature·Xueyong LiJiayang Li
Aug 18, 2000·Immunology and Cell Biology·A A RosenkranzA S Sobolev
Feb 25, 2003·Journal of Experimental Botany·Luis A del RíoBarbara A Zilinskas
Jan 31, 2013·Journal of Experimental Botany·Juan C Begara-MoralesFrancisco J Corpas
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·B RelićB Hohn
Oct 20, 1999·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·N Yamamoto, X W Deng
Jan 8, 2013·Trends in Plant Science·Olga ŠamajováJozef Šamaj
Mar 10, 2007·The Plant Journal : for Cell and Molecular Biology·Thirumala-Devi KannegantiSaskia A Hogenhout
Mar 1, 2006·Molecular Plant Pathology·Alexander KrichevskyVitaly Citovsky
Jun 21, 2002·The Journal of General Virology·Aletta KarsiesDenis Leclerc
Jun 21, 2005·Current Biology : CB·Kristoffer PalmaXin Li
Apr 26, 2014·Frontiers in Plant Science·Kentaro Tamura, Ikuko Hara-Nishimura
Sep 18, 2016·Journal of Experimental Botany·Juhong LiuLizhong Xiong
Aug 5, 2017·Journal of Cell Science·Francisco J Corpas, Juan B Barroso
Sep 30, 2000·British Journal of Nursing : BJN·K Dunne, V E Coates
Jul 17, 1998·Medicinal Research Reviews·D A JansS Huebner
May 5, 2020·The Plant Journal : for Cell and Molecular Biology·Chong ChenJae Bok Heo
Sep 26, 2019·Frontiers in Plant Science·Ramon ContrerasEnrique Rojo
Aug 1, 2004·The New Phytologist·Federica BrandizziDavid E Evans
Apr 10, 1999·Plant Physiology·H M Smith, N V Raikhel
Nov 13, 2001·Molecular Cell Biology Research Communications : MCBRC·A V Andreeva, M A Kutuzov
Jul 10, 1998·Virology·G R Whittaker, A Helenius

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.