Importin alpha1 (Rch1) mediates nuclear translocation of thioredoxin-binding protein-2/vitamin D(3)-up-regulated protein 1

The Journal of Biological Chemistry
Yumiko NishinakaJunji Yodoi

Abstract

Thioredoxin-binding protein-2 (TBP-2)/vitamin D(3) up-regulated protein 1 is an endogenous molecule interacting with thioredoxin (TRX), negatively regulating TRX function, and being implicated in the suppression of tumor development and metastasis. We found that TBP-2 ectopically expressed in the breast cancer cell line MCF-7 was localized predominantly in the nucleus exhibiting growth suppressive activity. The nuclear accumulation of endogenous TBP-2 protein was also demonstrated when the cells were treated with an anti-cancer drug, suberoylanilide hydroxamic acid. To investigate the mechanism underlying the nuclear localization, we performed a yeast two-hybrid screening and identified importin alpha(1) (Rch1) as a protein interacting with TBP-2. The physical interaction between TBP-2 and Rch1 was confirmed with a glutathione S-transferase pull-down assay. The interaction of TBP-2 was specific to Rch1 among other importin alpha subfamilies (Qip1 and NPI-1), and amino acids 1-227 of TBP-2 were sufficient for both the interaction with Rch1 and the nuclear localization, although there is no typical nuclear localization signal in this sequence. The expression of short interfering RNA of Rch1 suppressed suberoylanilide hydroxamic a...Continue Reading

References

May 18, 2000·Biochemical and Biophysical Research Communications·H YamanakaY Nakashima
Jun 8, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·E JunnI Choi
Oct 13, 2000·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Y Yoneda
Dec 26, 2001·Nature Genetics·Jackie S BodnarAldons J Lusis
Jan 10, 2002·Redox Report : Communications in Free Radical Research·Y NishinakaJ Yodoi
Aug 22, 2002·Proceedings of the National Academy of Sciences of the United States of America·Lisa M ButlerVictoria M Richon
Sep 6, 2002·The Journal of Biological Chemistry·Tsuyoshi HirotaYoshitaka Fukada
Apr 5, 2003·Pathology International·Shinya ToyokuniHiroshi Hiai

❮ Previous
Next ❯

Citations

Oct 6, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Cameron J WorldBradford C Berk
May 9, 2009·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Shin-ichi OkaJunichi Sadoshima
Jun 6, 2006·Journal of Molecular Histology·Shu-Chun TengLi Kao
Aug 4, 2009·Nature Chemical Biology·Tobias B DansenBoudewijn M T Burgering
Dec 2, 2010·Nature Communications·Eiji YoshiharaHiroshi Masutani
Apr 9, 2005·Cell Death and Differentiation·H MasutaniJ Yodoi
Jul 17, 2009·The Journal of Biological Chemistry·Parth PatwariRichard T Lee
Dec 5, 2009·The Journal of Biological Chemistry·Geetu SaxenaAnath Shalev
Sep 22, 2006·Antioxidants & Redox Signaling·Junichi Sadoshima
Oct 13, 2006·Antioxidants & Redox Signaling·Jun YoshiokaRichard T Lee
Apr 11, 2007·Antioxidants & Redox Signaling·Tetsuro Ago, Junichi Sadoshima
May 22, 2007·Antioxidants & Redox Signaling·Yuma HoshinoJunji Yodoi
Mar 10, 2010·Antioxidants & Redox Signaling·Young-Mi Go, Dean P Jones
Sep 21, 2011·Antioxidants & Redox Signaling·Oded N SpindelBradford C Berk
May 23, 2012·Antioxidants & Redox Signaling·Samuel LeeRichard T Lee
Dec 19, 2012·Antioxidants & Redox Signaling·Dler Faieeq Darweesh MahmoodMustapha Rouis
Aug 28, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Louise L DunnMartin K C Ng
Feb 22, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Oded N SpindelBradford C Berk
Nov 16, 2012·Journal of Molecular Endocrinology·Katherine A RobinsonMaria G Buse
Jan 17, 2012·Journal of Clinical Biochemistry and Nutrition·Hiroshi MasutaniJunji Yodoi
Feb 5, 2014·Biochemistry. Biokhimii︠a︡·S S ShishkinM A Kovaleva
Feb 7, 2015·The Biochemical Journal·Ruth A Pumroy, Gino Cingolani
Jul 25, 2013·Biochemical and Biophysical Research Communications·Hyun-Woo SuhInpyo Choi
Sep 16, 2015·Cell Communication and Signaling : CCS·Claudia LennickeBarbara Seliger
Nov 2, 2016·Journal of Biochemistry·Noriko Yasuhara, Penmetcha K R Kumar
Jul 21, 2016·Oxidative Medicine and Cellular Longevity·Benjamin J ForredPeter F Vitiello
Jan 5, 2005·Journal of Hepatology·Hiroaki OkuyamaJunji Yodoi
Aug 12, 2016·Molecular Neurobiology·Yousong SuDonghong Cui
Sep 30, 2006·Journal of Molecular and Cellular Cardiology·Tetsuro Ago, Junichi Sadoshima
Feb 22, 2005·Free Radical Biology & Medicine·Stephen LynnTing-Ting Huang
Nov 11, 2006·Seminars in Cancer Biology·Hajime NakamuraJunji Yodoi

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.