PMID: 6409963Aug 12, 1983Paper

Imprint immunofixation of electrofocused immunoglobulins

Journal of Immunological Methods
F Vartdal, B Vandvik

Abstract

An imprint immunofixation (IIF) technique for the characterization of electrofocused immunoglobulins (Ig) is described. Electrofocused proteins are blotted (imprinted) from the separating polyacrylamide gel to agarose gels by gel-to-gel overlays. The protein imprints are chemically fixed in the agarose gels with a solution of 46% methanol, 8% acetic acid and 46% water. The imprinted Ig are then identified radioimmunologically, using an indirect system with monoclonal mouse anti-human Ig antibodies in the first layer and 125I-labelled rabbit anti-mouse Ig in the second, followed by autoradiography. The method is sensitive and permits characterization of Ig in unconcentrated cerebrospinal fluid. By sequential imprinting, each separated specimen can be characterized for up to 10 separate antigenic determinants without loss of sensitivity.

References

Jul 1, 1974·Analytical Biochemistry·S C MarchP Cuatrecasas
Sep 1, 1965·Immunochemistry·G ManciniJ F Heremans

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Citations

Oct 1, 1983·Scandinavian Journal of Immunology·F Vartdal, P Bird
Apr 1, 1986·Infection and Immunity·M HarboeN Cruz

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