Imprinting of protein over silica nanoparticles via surface graft copolymerization using low monomer concentration
Abstract
Surface imprinting and adopting a nano-sized physical form are two effective approaches to overcome the template transfer difficulty within molecularly imprinted polymers and in particular advantageous to the imprinting of macromolecular structures like proteins. The surface protein-imprinted nanoparticles based on these two strategies are attractive for biosensor development. We here demonstrate a facile way for imprinting protein over nanoparticle supports. It was achieved simply via radical induced graft copolymerization of low concentration monomers on the surface of vinyl modified silica nanoparticles dispersed in aqueous media with lysozyme as a model protein. With a total monomer concentration of 0.4 wt%, less than tenth that employed conventionally, the possible gelation of the dispersion after polymerization was avoided and hence the unagglomerated imprinted particles could be readily collected. It was proved that thin polymer shells with imprinted sites had been formed over the support particles. In batch rebinding tests, the imprinted particles reached saturated adsorption within 5 min and exhibited significant specific recognition toward the template protein. The presented approach may be a versatile way for the fab...Continue Reading
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