Improved antibiotic resistance gene cassette for marker exchange mutagenesis in Ralstonia solanacearum and Burkholderia species

The Journal of Microbiology
Hae Young UmSeon-Woo Lee

Abstract

Marker exchange mutagenesis is a fundamental approach to understanding gene function at a molecular level in bacteria. New plasmids carrying a kanamycin resistance gene or a trimethoprim resistance gene were constructed to provide antibiotic resistance cassettes for marker exchange mutagenesis in Ralstonia solanacearum and many antibiotic-resistant Burkholderia spp. Insertion sequences present in the flanking sequences of the antibiotic resistance cassette were removed to prevent aberrant gene replacement and polar mutation during mutagenesis in wild-type bacteria. Plasmids provided in this study would be convenient for use in gene cassettes for gene replacement in other Gram-negative bacteria.

Citations

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Related Concepts

Shuttle Vectors
Trimethoprim
Recombination, Genetic
Gene Deletion
Burkholderia
Gram-Negative Bacteria
Gene Function
Burkholderia species
Plasmids
Mutagenesis Procedure

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