PMID: 9181457May 1, 1997Paper

Improved antibody targeting with interferon-alpha-2b conjugate

Journal of Immunotherapy
M L ThakurS Srivastava

Abstract

This investigation is based on a hypothesis that a biological response modifier, interferon-alpha-2b (IFN), when conjugated with a specific monoclonal antibody (mAb) and given to tumor-bearing animals before the administration of radiolabeled mAb, may not only augment the tumor uptake but may also impede the liver and blood uptake, because the mAb associated with the conjugate may block nonspecific hepatic binding sites and scavenge circulating antigens. ME 31.3 and anti-carcinoembryonic antigen (CEA) F-6 (IgG-2a) specific for human melanoma and colorectal carcinoma, respectively, were chosen as prototype mAbs and conjugated with IFN-alpha-2b for evaluation in athymic nude mice bearing respective experimental tumors. The mAb specificity for the tumor cell line was examined by binding assays and Kd values were determined to be 1.96 x 10(-9) M and 5.9 x 10(-9) M for ME 31.3 and anti-CEA F-6, respectively. Thirty micrograms of conjugate, prepared chemically and purified chromatographically (IFN-mAb:1:1), was administered intravenously to each animal and 3 h later followed by an intravenous injection of 20 micrograms F(ab')2 of corresponding mAb labeled with 300 muCi Tc-99m (1.5 Ci/mmol). Twenty-four hours later, in melanoma-bearin...Continue Reading

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.