Improved antimicrobial activity of Pediococcus acidilactici against Salmonella Gallinarum by UV mutagenesis and genome shuffling

Applied Microbiology and Biotechnology
Geon Goo HanYun-Jaie Choi

Abstract

Pediococcus acidilactici is a widely used probiotic, and Salmonella enterica serovar Gallinarum (SG) is a significant pathogen in the poultry industry. In this study, we improved the antimicrobial activity of P. acidilactici against SG using UV mutation and genome shuffling (GS). To improve antimicrobial activity against SG, UV mutagenesis was performed against wild-type P. acidilactici (WT), and five mutants showed improved antimicrobial activity. To further improve antimicrobial activity, GS was performed on five UV mutants. Following GS, four mutants showed improved antimicrobial activity compared with the UV mutants and WT. The antimicrobial activity of GS1 was highest among the mutants; however, the activity was reduced when the culture supernatant was treated with proteinase K, suggesting that the improved antimicrobial activity is due to a proteinous substance such as bacteriocin. To validate the activity of GS1 in vivo, we designed multi-species probiotics and performed broiler feeding experiments. Groups consisted of no treatment (NC), avilamycin-treated (PC), probiotic group 1 containing WT (T1), and probiotic group 2 containing GS1 (T2). In broiler feeding experiments, coliform bacteria were significantly reduced in ...Continue Reading

References

Mar 1, 1990·International Journal of Food Microbiology·P E Granum
May 1, 1989·The Journal of Applied Bacteriology·R Fuller
Oct 1, 1988·Applied and Environmental Microbiology·M J PucciP A Vandenbergh
Jan 1, 1987·Plasmid·L MorelliV Sgaramella
Jun 29, 2002·Nature Biotechnology·Ranjan PatnaikStephen del Cardayré
Mar 5, 2004·The Lancet Infectious Diseases·D BogaertP W M Hermans
Sep 30, 2004·International Journal of Food Microbiology·H M TimmermanA C Beynen
Apr 23, 2005·Veterinary Research·John E MoorePaul Whyte
Apr 17, 2007·Comparative Immunology, Microbiology and Infectious Diseases·Sunghyen LeeJ J Lin
Nov 23, 2007·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·G FamularoF Pirovano
Oct 11, 2008·Journal of Industrial Microbiology & Biotechnology·Dong-jian ShiKui-ming Wang
Mar 17, 2012·Therapeutic Advances in Gastroenterology·Theodoros Kelesidis, Charalabos Pothoulakis
Apr 3, 2016·Applied Microbiology and Biotechnology·Panagiotis Chanos, Tina Mygind
Mar 30, 2017·Peritoneal Dialysis International : Journal of the International Society for Peritoneal Dialysis·Karima FarhatFrans J van Ittersum

Citations

Oct 24, 2019·World Journal of Microbiology & Biotechnology·Ping YuTingting Yan
Sep 25, 2020·World Journal of Microbiology & Biotechnology·Liang ChenKe Bian

Datasets Mentioned

BETA
SRX2424928

Methods Mentioned

BETA
PCR

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.