Improved ATP supply enhances acid tolerance of Candida glabrata during pyruvic acid production

Journal of Applied Microbiology
Jingwen ZhouJian Chen

Abstract

A major problem in industrial fermentation of organic acids with micro-organisms is to ensure a suitable pH in the culture broth. To circumvent this problem, we investigated the effect of citrate, which is a widely used auxiliary energy co-substrate, on cell growth, organic acid production and pH homeostasis among extracellular environment, cytoplasm and vacuole, in the pyruvic acid production by Candida glabrata CCTCC M202019 under different pH conditions. Analysis of intracellular ATP regeneration, cytoplasmic and vacuolar pH values under different culture conditions points towards a relief of stress when C. glabrata is exposed to lower pH, if citrate is added. When 50 mmol l(-1) citrate was added to the culture medium, the intracellular ATP concentrations increased by 20·5% (pH 5·5), 20·4% (pH 5·0) and 39·3% (pH 4·5), and higher pH gradients among the culture broth, cell cytoplasm and vacuoles resulted. As a consequence, the cell growth and pyruvic acid production of C. glabrata CCTCC M202019 were significantly improved under pH 5·0 and 4·5. The acid tolerance of yeast can be improved by enhancing the ATP supply, which helps to maintain higher pH gradients in the system. The results presented here expand our understanding of...Continue Reading

References

Jan 1, 1975·Methods in Enzymology·A AustC H Suelter
Jan 1, 1975·Methods in Enzymology·E A Barnard
Mar 1, 1991·Journal of General Microbiology·C A Viegas, I Sá-Correia
Jan 1, 1990·Methods in Enzymology·S M Jazwinski
Sep 9, 1999·Bioscience, Biotechnology, and Biochemistry·K ZiegelbauerW Paffhausen
Nov 22, 2000·The Biochemical Journal·O J SomsenH V Westerhoff
Jun 29, 2002·Nature Biotechnology·Ranjan PatnaikStephen del Cardayré
Mar 17, 2005·Applied Microbiology and Biotechnology·M I TorinoG Font de Valdez
Jul 6, 2005·Current Opinion in Microbiology·Rupinder KaurBrendan P Cormack
Oct 20, 2005·Journal of Bioscience and Bioengineering·K SatoT Ohba
Oct 22, 2005·Applied Microbiology and Biotechnology·H D MortensenN Arneborg
Jan 21, 2006·The Journal of Biological Chemistry·Sergio Padilla-López, David A Pearce
Dec 1, 1996·Applied and Environmental Microbiology·G M CookJ Wiegel
Jun 14, 2006·The Journal of Biological Chemistry·Yan ZhangAndrew Dancis
Aug 4, 2006·Applied and Environmental Microbiology·Minoska ValliDiethard Mattanovich
Aug 9, 2006·Biotechnology and Bioengineering·M CánovasJ L Iborra
Oct 18, 2006·Applied Microbiology and Biotechnology·Hiroyuki HidaYasuhiro Yamada
Jul 3, 2007·Applied and Environmental Microbiology·Juan ZhangJian Chen
Jul 17, 2007·Biotechnology and Applied Biochemistry·Pingying WeiNing Jiang
Sep 28, 2007·The International Journal of Biochemistry & Cell Biology·Yong-Tao XiaoJian-Zhong Shao
Oct 24, 2007·Eukaryotic Cell·Todd B ReynoldsGerald R Fink
Dec 25, 2007·Applied and Environmental Microbiology·Claudia SánchezHelena Santos
Jan 11, 2008·Proteomics·Pia SchmidtAlistair J P Brown
Jan 15, 2008·Trends in Biotechnology·Michael SauerPaola Branduardi
Apr 1, 2008·Eukaryotic Cell·Héloïse MullerCécile Fairhead
Apr 15, 2008·Archives of Biochemistry and Biophysics·Kevin C JefferiesMichael Forgac
May 27, 2008·The Journal of Biological Chemistry·Gloria A Martínez-Muñoz, Patricia Kane
Jun 10, 2008·Applied and Environmental Microbiology·Henrik Dam MortensenNils Arneborg
Nov 26, 2008·Biotechnology Advances·Jingwen ZhouJian Chen
Feb 11, 2009·Proceedings of the National Academy of Sciences of the United States of America·Silvia PolákováJure Piskur
Aug 12, 2009·Journal of Applied Microbiology·H DriciD Atlan
Oct 3, 2009·Journal of Applied Microbiology·L WaddingtonM Kalmokoff

❮ Previous
Next ❯

Citations

Jul 23, 2013·Applied Microbiology and Biotechnology·Dahui WangWei Gongyuan
Dec 15, 2015·Microbial Cell Factories·Kiyotaka Y Hara, Akihiko Kondo
Oct 21, 2015·Applied Biochemistry and Biotechnology·Ying LiuPingkai Ouyang
Sep 18, 2015·Microbial Cell Factories·Eugene FletcherJens Nielsen
Sep 6, 2014·Bioprocess and Biosystems Engineering·Ying LiuPingkai Ouyang
Sep 4, 2012·Biotechnology Advances·Qing XuJianping Wen
Sep 9, 2015·Applied Microbiology and Biotechnology·Da-Hui WangBin Qi
Jan 24, 2012·International Journal of Food Sciences and Nutrition·Siok-Koon Yeo, Min-Tze Liong
Sep 22, 2017·FEMS Yeast Research·Kiyotaka Y HaraAkihiko Kondo
Nov 28, 2019·Applied Microbiology and Biotechnology·Ningzi Guan, Long Liu
Apr 18, 2020·Applied Microbiology and Biotechnology·Mingzhu HuangXuelan Chen
Aug 2, 2020·Journal of Industrial Microbiology & Biotechnology·Hongmei HanJingwen Zhou

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Epigenetics Insights from Twin Studies

Find the latest research on epigenetics and twin studies here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.