Improved axenic hydroponic whole plant propagation for rapid production of roots as transformation target tissue

Plant Methods
Kyle Benzle, Katrina Cornish

Abstract

Plant roots are used as an efficient target tissue for plant transformation assays. In root propagable species transformed roots are able to regenerate into whole plants without the addition of exogenous hormones, thus avoiding somaclonal variation associated with many plant transformation protocols. Plants grown in soil or soilless solid medium have roots that tend to be extremely delicate and are difficult to sterilize in advance of plant transformation experiments. Axenic tissue culture plants grown on semi-solid media are slow to produce large amounts of biomass compared to plants grown in solution-based media. Seeds were germinated and grown for 14 days on half-strength semi-solid Murashige and Skoog medium containing 1% sucrose. Seedlings were then transferred to Magenta™ GA7 vessels containing either liquid or semi-solid ½ MS medium with 0.25, 0.5, 1, 2 or 3% sucrose. In the hydroponics (liquid medium) treatments, expanded clay balls were used to anchor seedlings. Hydroponic vessels were fitted with a sterile air aeration hose and filled ¾ full (100 mL) with liquid ½ MS media. Liquid media were replaced after 7 days. All plants were grown under fluorescent lights for 14 days. We have developed an improved axenic hydropon...Continue Reading

References

Apr 1, 1968·Experimental Cell Research·O L GamborgK Ojima
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·R. G. Birch
Feb 23, 2010·BMC Biochemistry·Thomas SchmidtChristian Schulze Gronover
Oct 30, 2013·The International Journal of Developmental Biology·Sylvester AnamiMieke Van Lijsebettens
Mar 22, 2014·BMC Plant Biology·Fulgencio Alatorre-CobosLuis Herrera-Estrella

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