Improved beta-lactam acylases and their use as industrial biocatalysts

Current Opinion in Biotechnology
Charles F Sio, Wim J Quax

Abstract

Whereas the beta-lactam acylases are traditionally used for the hydrolytic processing of penicillin G and cephalosporin C, new and mutated acylases can be used for the hydrolysis of alternative fermentation products as well as for the synthesis of semisynthetic beta-lactam antibiotics. Three-dimensional structural analyses and site-directed mutagenesis studies have increased the understanding of the catalytic mechanism of these enzymes. The yield of hydrolysis and synthesis has been greatly improved by process design, including immobilization of the enzyme and the use of alternative reaction media. Significant advances have also been made in the resolution of racemic mixtures by means of stereoselective acylation/hydrolysis using beta-lactam acylases.

References

May 31, 1972·Journal of the American Chemical Society·T TakahashiM Isona
Jan 19, 1995·Nature·H J DugglebyP C Moody
Nov 18, 2000·Structure·Y KimW G Hol
Feb 22, 2002·Biochemical and Biophysical Research Communications·Raquel Torres-GuzmánCarmen Acebal
Aug 29, 2002·The Journal of Biological Chemistry·Linda G OttenWim J Quax
Sep 17, 2002·European Journal of Biochemistry·Charles F SioWim J Quax
Oct 16, 2002·Biotechnology and Bioengineering·Luciana R B GonçalvesRoberto C Giordano
Oct 19, 2002·Journal of Biotechnology·R V UlijnA E M Janssen
Jan 22, 2003·Applied Microbiology and Biotechnology·M ArroyoM Pilar Castillón
Apr 8, 2003·Applied Microbiology and Biotechnology·R P Elander
Jun 27, 2003·Applied Microbiology and Biotechnology·K PlháckováP Kyslík
Sep 3, 2003·European Journal of Biochemistry·Wynand B L AlkemaDick B Janssen
Oct 10, 2003·The Journal of Biological Chemistry·Jongchul YoonYoungsoo Kim
Dec 19, 2003·Biochemical and Biophysical Research Communications·Charles F SioWim J Quax
Dec 31, 2003·Biochemical and Biophysical Research Communications·Wei ZhangWeihong Jiang
Jun 3, 2004·Chembiochem : a European Journal of Chemical Biology·Linda G OttenWim J Quax

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Citations

Nov 14, 2006·Applied and Environmental Microbiology·Tamara MontesJosé M Guisán
Feb 24, 2006·Infection and Immunity·Charles F SioWim J Quax
Sep 12, 2007·BMC Biotechnology·Davide A CecchiniAlessandra M Albertini
Jan 22, 2014·Applied Microbiology and Biotechnology·Helena MarešováPavel Kyslík
Nov 29, 2014·Critical Reviews in Biotechnology·Vellore Sunder AvinashCheravakkattu Gopalan Suresh
Oct 23, 2010·Bioresource Technology·Junqi ZhaoShenlin Zhu
Aug 13, 2015·The Journal of Membrane Biology·Malinidevi RamanathanRajendran Lakshmanan
Sep 26, 2009·Microbiology·Pol Nadal JimenezWim J Quax
Jan 27, 2015·Applied Microbiology and Biotechnology·Ismail EşAndré Corrêa Amaral
Jun 2, 2009·Journal of Biotechnology·Joanna KrzeslakWim J Quax
Jun 24, 2017·Chemical Communications : Chem Comm·Xiaoqiang HuangYushan Zhu
Aug 8, 2006·Biotechnology Journal·Karen M PolizziAndreas S Bommarius
Jul 16, 2010·Biotechnology and Bioengineering·Susana M S A BernardinoLuís P Fonseca
Jul 1, 2006·Critical Reviews in Biotechnology·Vijay Chintaman Sonawane
Jan 1, 2010·Biotechnology & Genetic Engineering Reviews·Marco Van Den BergIda Vander Keli
Nov 9, 2012·Natural Product Reports·Refaat B HamedChristopher J Schofield
Jul 5, 2017·Frontiers in Microbiology·Putri D UtariWim J Quax
Jul 20, 2021·Journal of Industrial Microbiology & Biotechnology·Riccardo IacovelliArnold J M Driessen
Aug 9, 2012·Journal of the American Chemical Society·Florian RichterDavid Baker
Oct 13, 2009·Journal of Photochemistry and Photobiology. B, Biology·Atul KumarArchana Pundle

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