Improved beta-thujaplicin production in Cupressus lusitanica suspension cultures by fungal elicitor and methyl jasmonate

Applied Microbiology and Biotechnology
J ZhaoK Sakai

Abstract

Production of a novel antimicrobial tropolone, beta-thujaplicin, in Cupressus lusitanica suspension cultures was studied by using a variety of chemicals and fungal elicitors. Sodium alginate, chitin, and methyl jasmonate resulted in 2-, 2.5-, and 3-fold higher beta-thujaplicin production, respectively, than in the control. Significantly improved beta-thujaplicin production (187 mg l(-1)) was obtained using a high cell density (180-200 g l(-1)) and fungal elicitor treatment [10 mg (g fresh cells)(-1)] in a production medium with a high ferrous ion concentration (0.3 mM). This improved volumetric productivity was 3- to 4-fold higher than obtained under standard conditions. A synergistic effect of fungal elicitor and ferrous ion on beta-thujaplicin production was also suggested by our study. Plant cell culture technology is a promising alternative for producing a large variety of secondary metabolites that are widely used as food additives, pharmaceuticals, and dairy products (Verpoorte et al. 1999). Thus, beta-thujaplicin production by plant cell cultures was developed with the goal of commercial application (Berlin and Witte 1988; Itose and Sakai 1997; Ono et al. 1998). However, the production of beta-thujaplicin by plant cell c...Continue Reading

Citations

Jan 5, 2005·Applied Microbiology and Biotechnology·Zhi-Gang QianJian-Jiang Zhong
Aug 7, 2013·Plant Foods for Human Nutrition·Elena AlgarF Javier Gutiérrez-Mañero
Oct 9, 2012·Journal of Agricultural and Food Chemistry·Elena AlgarBeatriz Ramos-Solano
Aug 1, 2014·Journal of Environmental Sciences (China)·Junyi HuYingqian Xu
Feb 6, 2008·Natural Product Reports·Ronald Bentley
Dec 25, 2009·Annals of Tropical Medicine and Parasitology·C W LiaoC K Fan
Jul 7, 2018·Beilstein Journal of Organic Chemistry·Arif DastanNurullah Saracoglu
Apr 26, 2005·Biotechnology Advances·Jian ZhaoRobert Verpoorte

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