Improved between-laboratory agreement for specific protein assays in serum following introduction of a common reference preparation (SPS-01) demonstrated in an external quality assessment scheme

Clinica Chimica Acta; International Journal of Clinical Chemistry
R E ChambersJ T Whicher

Abstract

Between-laboratory agreement for 6 specific protein assays was better in two surveys (total 8 specimens) in which a calibration material was provided than in the preceding five surveys (20 specimens) in a national external quality assessment scheme. CVs for immunoglobulins G, A and M (200 participants) and alpha 1-antitrypsin and complement components C3 and C4 (70 participants) were reduced from 18.3% (range 11.6-23.8%) to 10.7% (range 7.5-13.6%). This improvement was not due to changes in within-laboratory precision or in the concentrations of protein surveyed. Improvement was maintained in the following four surveys (3 normal and 5 pathological sera) without calibration material for immunoglobulins G and A and for alpha 1-antitrypsin. Agreement for immunoglobulin M, C3 and C4 returned to values close to the original CVs, but subsequently improved. The role of a common calibration material in improving between-laboratory agreement is discussed.

Citations

May 11, 2013·Analytical and Bioanalytical Chemistry·Claudia Swart
Oct 22, 2010·Clinical Chemistry and Laboratory Medicine : CCLM·Giampaolo MerliniIFCC Committee on Plasma Proteins (C-PP)
Apr 12, 2005·Clinical Biochemistry·Jean DubePierre Douville
Oct 20, 1990·BMJ : British Medical Journal·D Slater
Nov 21, 1997·Annals of Clinical Biochemistry·S R Goodall

Related Concepts

Prolastin
Serum Proteins
Complement C3 precursor
Complement C4, Precursor
Immunoglobulins
Laboratory
Standardization

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