Improved cell typing by charge-state deconvolution of matrix-assisted laser desorption/ionization mass spectra

Rapid Communications in Mass Spectrometry : RCM
Jon G WilkesAlexandre A Shvartsburg

Abstract

Robust, specific, and rapid identification of toxic strains of bacteria and viruses, to guide the mitigation of their adverse health effects and optimum implementation of other response actions, remains a major analytical challenge. This need has driven the development of methods for classification of microorganisms using mass spectrometry, particularly matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), that allows high-throughput analyses with minimum sample preparation. We describe a novel approach to cell typing based on pattern recognition of MALDI mass spectra, which involves charge-state deconvolution in conjunction with a new correlation analysis procedure. The method is applicable to both prokaryotic and eukaryotic cells. Charge-state deconvolution improves the quantitative reproducibility of spectra because multiply charged ions resulting from the same biomarker attaching a different number of protons are recognized and their abundances are combined. This allows a clearer distinction of bacterial strains or of cancerous and normal liver cells. Improved class distinction provided by charge-state deconvolution was demonstrated by cluster spacing on canonical variate score charts and by correlation ...Continue Reading

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Citations

Jul 19, 2008·Annual Review of Analytical Chemistry·Plamen A Demirev, Catherine Fenselau
Dec 1, 2006·Journal of Mass Spectrometry : JMS
Aug 23, 2008·Journal of Mass Spectrometry : JMS·Plamen A Demirev, Catherine Fenselau
Dec 5, 2009·Rapid Communications in Mass Spectrometry : RCM·Dariya I MalyarenkoDennis M Manos
Feb 29, 2016·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J VeenemansJ Kluytmans

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