Improved chemotherapy against breast cancer through immunotherapeutic activity of fucoidan decorated electrostatically assembled nanoparticles bearing doxorubicin

International Journal of Biological Macromolecules
Vivek K PawarManish K Chourasia

Abstract

Immunotherapeutic nanoparticles (NPs) could be a viable option for delivering cytotoxic agents in a manner which suppresses their toxic manifestations. Doxorubicin (DOX) loaded NPs were prepared using fucoidan (FCD), an immunomodulatory polysaccharide and evaluated against cancer. FCD was electrostatically assembled with cationic polyethylenimine (PEI) through intermolecular electrostatic interactions to develop an immunomodulatory platform to deliver DOX. FCD NPs offered improved cytotoxicity (2.64 folds), cell cycle arrest in G1-S phase (34.65%) and apoptosis (66.12%) in tumor cells compared to free DOX. The enhanced apoptosis was due to raised mitochondrial depolarization (88.00%). In vivo anticancer activity in 4T1 induced tumor bearing BALB/c mice demonstrated a 2.95 folds enhanced efficacy of NPs. Importantly, NPs treatment generated an immunotherapeutic response indicated by gradual increment of the plasma IL-12 levels and reversed polarization of tumor associated macrophages (TAMs) towards M1 subtype. Furthermore, pharmacokinetic study suggested that NPs administration in tumor infested mice caused serum DOX levels to vary in a biphasic pattern, with twin peaks occurring at 1 h and 6 h which help in maintaining preferen...Continue Reading

Citations

Oct 12, 2019·Marine Drugs·Helen J FittonSamuel N Karpiniec
Oct 8, 2019·Current Drug Metabolism·Phuong H L Tran, Thao T D Tran
Aug 8, 2019·Marine Drugs·Aleksandra CitkowskaKatarzyna Winnicka
May 2, 2020·Marine Drugs·María Elena ReyesPriscilla Brebi
Oct 23, 2020·International Journal of Biological Macromolecules·Kun-Ying LuEr-Yuan Chuang
Dec 20, 2020·International Journal of Biological Macromolecules·Samar M EtmanOssama Y Abdallah
Jan 30, 2021·Life Sciences·Nastaran HashemzadehJaleh Barar
Jul 28, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jie ZhangJufang Chi
Aug 10, 2021·Nanomedicine·Catarina OliveiraTiago H Silva
Aug 18, 2021·Food Research International·Yuezhen OuyangChao Zhao

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