PMID: 6988328Feb 1, 1980Paper

Improved conditions for murine epidermal cell culture

In Vitro
S M FischerT J Slaga

Abstract

An improved method for cultivating newborn mouse epidermal cells has been developed that increases the longevity, epithelial nature and efficiency of cell-line establishment. The use of Super Medium, an enriched Waymouth's formulation, increased proliferation for long periods of time, as did incubation at 31 degrees C rather than 37 degrees C. The fetal bovine serum requirement was found to be reduced at the lower temperature. An increase in labeling indices was seen when epidermal growth factor (EGF) or the cyclic nucleotides were added and the presence of EGF receptors was determined. Of the prostaglandins (PG) examined, PGE1 and PGE2 produced the greatest increase in DNA synthesis. The PG precursors, arachidonic and 8,11,14-eicosatrienoic acid, were also greatly stimulatory. The use of a lethally irradiated 3T3 feeder layer at 31 degrees C proved superior in maintenance of an epithelial morphology. Subculturable cell lines were established much more readily and reproducibly in carcinogen-treated cultures grown under the improved conditions.

References

Mar 15, 1974·Experimental Cell Research·D R ThomasB M Jaffe
Aug 1, 1974·The Journal of Investigative Dermatology·N E Fusenig, P K Worst
May 1, 1974·Experimental Cell Research·S H Yuspa, C C Harris
Dec 1, 1970·The Journal of Investigative Dermatology·S H YuspaR R Bates
Jun 1, 1971·The Journal of Investigative Dermatology·F L Vaughan, I A Bernstein
Mar 1, 1971·The Journal of Investigative Dermatology·M A Karasek, M E Charlton
Aug 1, 1971·Die Naturwissenschaften·N E Fusenig
Jan 1, 1963·The Journal of Investigative Dermatology·S COHEN, G A ELLIOTT
Oct 1, 1964·The Journal of Experimental Zoology·N K WESSELLS

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Citations

Jan 1, 1989·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S M FischerK E Patrick
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·V Solanki, T J Slaga
Jun 26, 1999·The Journal of Investigative Dermatology·B HagerP Fleckman
May 1, 1982·Experimental Cell Research·S Robine-LeonD Paulin
Sep 1, 1980·Experimental Cell Research·D R MillerT J Slaga
Nov 1, 1991·The American Journal of Physiology·F H KruszewskiR W Tucker

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