PMID: 6409964Aug 12, 1983Paper

Improved conditions for the production of monoclonal antibodies to carcinogen-modified DNA, for use in enzyme-linked immunosorbent assays (ELISA)

Journal of Immunological Methods
P J HertzogR C Garner

Abstract

The methodology for the production of monoclonal antibodies to chemical carcinogen-modified DNA has been improved to provide high yields of hybridomas, using guanine-imidazole ring-opened aflatoxin B1-modified DNA as an example (iro-AFB1 DNA). The percentage of immunised mice which responded to iro-AFB1 DNA-protein immunisation and the number of specific hybridomas produced was dependent on the level of modification of DNA. One in three BALB/c mice had detectable (but low) antibody titre when 0.3% modified iro-AFB1 DNA was used and this yielded 2 specific hybridomas, whereas all mice responded at reasonable titres and 6 specific hybridomas were obtained when 3% modified iro-AFB1 DNA was used. Other factors found to improve the number and titre of mice responding to immunisation and the yield of hybridomas were: KLH greater than BSA as carrier protein, C57 BL/6 X BALB/c F1 greater than BALB/c mice for antibody production, fusion success and ascites growth. The conditions limiting the sensitivity and reproducibility of an enzyme-linked immunosorbent assay (ELISA) using these monoclonal antibodies with beta-galactosidase-linked sheep F(ab')2 anti-mouse IgG as the second antibody were also tested. Present experience with AFB1 and o...Continue Reading

References

Nov 10, 1977·Nature·M C PoirierS Blobstein
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·A HaugenC C Harris
Jan 1, 1981·Journal of Immunological Methods·P T Strickland, J M Boyle
Jul 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·B F ERLANGER, S M BEISER

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Citations

Jul 24, 1998·Mutation Research·R C Garner
Sep 21, 2012·Acta Crystallographica. Section D, Biological Crystallography·Anna Bujacz
Nov 3, 2017·Acta Crystallographica. Section D, Structural Biology·Anna BujaczPiotr Neumann
May 30, 1991·International Journal of Cancer. Journal International Du Cancer·P J HertzogA W Linnane
Mar 1, 1985·Biochimie·M Leng

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