Improved crystallization and diffraction of caffeine-induced death suppressor protein 1 (Cid1)

Acta Crystallographica. Section F, Structural Biology Communications
Luke A YatesRobert J C Gilbert

Abstract

The post-transcriptional addition of uridines to the 3'-end of RNAs is an important regulatory process that is critical for coding and noncoding RNA stability. In fission yeast and metazoans this untemplated 3'-uridylylation is catalysed by a single family of terminal uridylyltransferases (TUTs) whose members are adapted to specific RNA targets. In Schizosaccharomyces pombe the TUT Cid1 is responsible for the uridylylation of polyadenylated mRNAs, targeting them for destruction. In metazoans, the Cid1 orthologues ZCCHC6 and ZCCHC11 uridylate histone mRNAs, targeting them for degradation, but also uridylate microRNAs, altering their maturation. Cid1 has been studied as a model TUT that has provided insights into the larger and more complex metazoan enzyme system. In this paper, two strategies are described that led to improvements both in the crystallogenesis of Cid1 and in the resolution of diffraction by ∼1.5 Å. These advances have allowed high-resolution crystallographic studies of this TUT system to be initiated.

References

Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Aug 12, 2004·FEBS Letters·Ruslan AphasizhevLarry Simpson
Nov 6, 2004·Science·Binzhang Shen, Howard M Goodman
May 26, 2005·Protein Expression and Purification·F William Studier
Dec 22, 2005·Acta Crystallographica. Section D, Biological Crystallography·Philip Evans
Dec 22, 2005·Acta Crystallographica. Section D, Biological Crystallography·Zygmunt S Derewenda, Peter G Vekilov
Dec 27, 2006·Journal of Molecular Biology·Jason StagnoRuslan Aphasizhev
Mar 14, 2007·Molecular and Cellular Biology·Olivia S RisslandChris J Norbury
May 12, 2009·Nature Structural & Molecular Biology·Olivia S Rissland, Chris J Norbury
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read
Aug 29, 2009·Nature Structural & Molecular Biology·John P HaganRichard I Gregory
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Wolfgang Kabsch
Aug 23, 2011·Methods : a Companion to Methods in Enzymology·Louise E Bird
May 23, 2012·Structure·Paola Munoz-TelloStéphane Thore
Jul 4, 2012·Nature Structural & Molecular Biology·Luke A YatesRobert J C Gilbert
Aug 14, 2012·Nucleic Acids Research·Bradley M LundeAnton Meinhart
Apr 30, 2013·Cell·Luke A YatesRobert J C Gilbert
May 28, 2013·The Journal of Biological Chemistry·Christopher P Lapointe, Marvin Wickens
Aug 31, 2013·Nature Reviews. Molecular Cell Biology·Chris J Norbury
Jun 11, 2014·Acta Crystallographica. Section D, Biological Crystallography·Christian X Weichenberger, Bernhard Rupp

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Citations

Feb 5, 2019·Nucleic Acids Research·Christina Z ChungIlka U Heinemann

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Methods Mentioned

BETA
size-exclusion chromatography
X-ray

Software Mentioned

XDS
SCALA
xia

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