Improved Cuff Technique and Intraoperative Detection of Vascular Complications for Hind Limb Transplantation in Mice

Transplantation Direct
Minhyung KimJoseph J Skitzki

Abstract

Vascularized composite tissue allotransplantation (VCA) from a cadaveric donor has now become a clinical reality and the treatment modality of choice for patients with devastating injuries, deformities, and complex tissue defects. However, many VCA patients experience severe toxicities due to the strong immunosuppression required secondary to high antigenicity of the grafts. To improve immunosuppressive protocols for VCA, feasible and reliable preclinical models are necessary. The purpose of this study was to introduce new techniques to an established preclinical VCA model to accelerate future investigations. C57BL/6 (H-2b) and BALB/c (H-2d) mice were used to perform VCA as recipients and donors, respectively. Surgery time, success rate, associated complications, and mortality were analyzed. Blood flow in grafts was interrogated with laser speckle image (LSI). A nonsuture cuff technique was used with the abdominal aorta for end-to-end anastomosis. The cuff technique demonstrated efficiency for donor surgery (52 ± 10 minutes for donor vs. 45 ± 8 minutes for recipient surgery). Successful revascularization was achieved in 27 (90%) of 30 transplants. The majority of surgical complications occurred within 48 hours including artery ...Continue Reading

References

Jun 1, 1978·The Journal of Surgical Research·R I Shapiro, F B Cerra
Nov 18, 1998·Microsurgery·B C Cooley, R Daley
Jun 9, 2001·Journal of Reconstructive Microsurgery·T H TungS E Mackinnon
Jun 29, 2001·Lancet·J M DubernardN Hakim
Mar 22, 2002·Transplantation·John H BarkerClaudio Maldonado
Dec 24, 2002·Annals of Surgery·François PetitW P Andrew Lee
Jul 18, 2006·Lancet·Bernard DevauchelleJean-Michel Dubernard
Nov 1, 2007·Seminars in Plastic Surgery·Aleksandra Klimczak, Maria Siemionow
Nov 1, 2007·Seminars in Plastic Surgery·Maria Siemionow, Yalcin Kulahci
Nov 6, 2010·Transplantation·Palmina PetruzzoChristian Dumontier
Oct 11, 2011·Transplant International : Official Journal of the European Society for Organ Transplantation·Theresa HautzStefan Schneeberger
Dec 20, 2012·Clinical & Developmental Immunology·Kadiyala V RavindraSuzanne T Ildstad
Mar 27, 2013·Archives of Plastic Surgery·Seok Chan Eun
Jan 29, 2014·Plastic and Reconstructive Surgery·Cheng-Hung LinGerald Brandacher
Aug 13, 2015·Transplant International : Official Journal of the European Society for Organ Transplantation·Maximilian KueckelhausStefan G Tullius

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Citations

Apr 17, 2020·Transplant International : Official Journal of the European Society for Organ Transplantation·Yinan GuoGerald Brandacher

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Methods Mentioned

BETA
gene knockout
dissecting
dissection
biopsy

Software Mentioned

FireWire
MoorFLPI
MoorFLPI Measurement

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