Improved detection and comparative sizing of human chromosomal telomeres in situ

Chromosoma
K Krejcí, J Koch

Abstract

Telomeric length dynamics are thought to play an important role both in the processes of cellular aging and cancer progression. We have revised the primed in situ (PRINS) labeling technique to allow an estimation of the relative length of individual telomeres. We illustrate the applicability of the approach by demonstrating different telomeric sizes not only between blood lymphocytes from a young and an old donor, but also among bone marrow cells from hematological cancer patients. In the latter case we found general variations in telomeric sizes as well as individual telomeric variations that would have escaped detection by other methods. An interesting finding was the selective expansion of a single telomere within a specific subset of cells.

Citations

Nov 7, 2002·Cancer Genetics and Cytogenetics·Nedime SerakinciJørn Koch
Mar 22, 2006·BMC Biotechnology·Robert HasterokTim Langdon
Mar 20, 2014·PloS One·Andrea Cukusic KalajzicIvica Rubelj
Feb 28, 2003·Pharmacogenomics·Rossa W K Chiu, Y M Dennis Lo
Sep 7, 2006·Cytogenetic and Genome Research·F PellestorS Hamamah
Aug 10, 2000·Neoplasia : an International Journal for Oncology Research·K KrejcíJ Koch
Feb 24, 2006·Mutation Research·Alejandro D Bolzán, Martha S Bianchi
Feb 14, 2006·European Journal of Medical Genetics·Sukru PalanduzJørn Koch
Dec 8, 2004·Annales de génétique·Petra Paulasova, Franck Pellestor
Feb 1, 2003·American Journal of Medical Genetics. Part C, Seminars in Medical Genetics·Jie Xu, Zhong Chen
Jan 19, 2010·Experimental Gerontology·Nikolina Skrobot VidacekIvica Rubelj
Mar 3, 1999·Nature Biotechnology·N Serakinci, J Koch
May 8, 2003·European Journal of Human Genetics : EJHG·Dan-Oscar AntsonMats Nilsson
Sep 5, 2008·Current Protocols in Cytometry·Jørn Koch
Apr 23, 2008·Current Protocols in Human Genetics·Jørn Koch

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