PMID: 11897797Mar 19, 2002Paper

Improved detection of apoptotic cells in archival paraffin sections: immunohistochemistry using antibodies to cleaved caspase 3

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Allen M Gown, Mark C Willingham

Abstract

Apoptosis has gained central importance in the study of many biological processes, including neoplasia, neurodegenerative diseases, and development. One of the limitations of many studies is the difficulty of specifically identifying individual apoptotic cells. Of the many specific methods developed to detect apoptotic cells, most are not applicable to histological sections of archival paraffin-embedded tissues. Recently, advances in the understanding of the molecular events in apoptosis have led to the realization that caspase activation is by far the most specific indicator of this cell suicide mechanism. Several publications have reported the development of antibodies directed at neoepitopes that are generated in various substrates through the action of caspases. One of these is that present on activated caspase 3, a ubiquitously distributed caspase that is a main effector caspase of the apoptotic cascade within cells. This study demonstrates the utility of using a recently commercially available antibody to cleaved caspase 3 in archival paraffin sections, suggesting that this may be a highly specific and sensitive method generally applicable to many studies of archival material.

References

Sep 1, 1996·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A NegoescuE Brambilla
Jul 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J A CollinsM C Willingham
Jan 23, 1999·Cell Death and Differentiation·A SrinivasanK J Tomaselli
Aug 17, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M C Willingham
Mar 21, 2001·Apoptosis : an International Journal on Programmed Cell Death·P R WebbJ M Lord
Jun 19, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K L BarrettM C Willingham

❮ Previous
Next ❯

Citations

Nov 15, 2007·Histochemistry and Cell Biology·Douglas J TaatjesRalph C Budd
Mar 27, 2004·Virchows Archiv : an International Journal of Pathology·Andrej CörNina Gale
Oct 6, 2005·Virchows Archiv : an International Journal of Pathology·Nina ZidarDusan Stajer
May 11, 2005·Neuroscience Letters·Joze PizemMara Popovic
Aug 7, 2010·Cell Death and Differentiation·A Hedrych-OziminaI Haase
May 25, 2004·Nature Genetics·José A CostoyaPier Paolo Pandolfi
Nov 6, 2009·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Kumi O KurodaNiall P Murphy
Jun 4, 2011·Reproduction, Fertility, and Development·Myoungkun Jeoung, Phillip J Bridges
Aug 18, 2012·Tissue Engineering. Part a·Pablo García-MirandaAnunciación A Ilundain
Dec 24, 2005·Critical Care Medicine·Vincenzo LionettiV Marco Ranieri
Dec 20, 2007·Applied Immunohistochemistry & Molecular Morphology : AIMM·Rita MachaalaniKaren A Waters
Mar 16, 2005·The American Journal of Surgical Pathology·Loren E ClarkeKlaus F Helm
Nov 30, 2011·TheScientificWorldJournal·Ljubinka Jankovic-VelickovicVladisav Stefanovic
Oct 31, 2006·Journal of Clinical Pathology·A S-Y Leong, T Y-M Leong
Feb 3, 2006·The Journal of Clinical Investigation·Yasushi MukaiJames K Liao
Nov 26, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Aude BressenotFrançois Plénat
Jan 17, 2008·PloS One·Jo N FlemingStephen M Schwartz
Apr 25, 2008·Journal of Clinical and Experimental Hematopathology : JCEH·Jia WangFusayoshi Murata
Nov 1, 2013·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Elisabeth J PechrigglHelga Fritsch
Nov 10, 2013·The Journal of Veterinary Medical Science·Jing SunSatoshi Furukawa
Nov 20, 2012·Future Medicinal Chemistry·Atsushi KittakaTai C Chen
Jan 18, 2014·Journal of Medicinal Food·Omar M E Abdel-SalamAmany A Sleem
Jun 21, 2014·Analytical and Bioanalytical Chemistry·Eva AdamováKarel Klepárník
Oct 9, 2013·Journal of Immunotoxicology·Dalia M Abd El MottelebAhmed M Mohamed
Jan 21, 2016·Oncology Letters·Rina KatoIchio Fukasawa
Sep 22, 2015·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Vinzent N SpetzlerMarkus Selzner
Apr 17, 2015·Liver International : Official Journal of the International Association for the Study of the Liver·Danielle AdebayoRajiv Jalan
Oct 2, 2012·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Sasa SopkaIrmgard Classen-Linke
Oct 9, 2012·Animal Reproduction Science·Eddy KaoTrish Berger
Sep 25, 2012·Molecular and Cellular Endocrinology·Yoav ElkisUri Nir

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis