Improved detection of Burkholderia pseudomallei from non-blood clinical specimens using enrichment culture and PCR: narrowing diagnostic gap in resource-constrained settings

Tropical Medicine & International Health : TM & IH
Chaitanya TellapragadaChiranjay Mukhopadhyay

Abstract

To evaluate the diagnostic utility of enrichment culture and PCR for improved case detection rates of non-bacteraemic form of melioidosis in limited resource settings. Clinical specimens (n = 525) obtained from patients presenting at a tertiary care hospital of South India with clinical symptoms suggestive of community-acquired pneumonia, lower respiratory tract infections, superficial or internal abscesses, chronic skin ulcers and bone or joint infections were tested for the presence of Burkholderia pseudomallei using conventional culture (CC), enrichment culture (EC) and PCR. Sensitivity, specificity, positive and negative predictive values of CC and PCR were initially deduced using EC as the gold standard method. Further, diagnostic accuracies of all the three methods were analysed using Bayesian latent class modelling (BLCM). Detection rates of B. pseudomallei using CC, EC and PCR were 3.8%, 5.3% and 6%, respectively. Diagnostic sensitivities and specificities of CC and PCR were 71.4, 98.4% and 100 and 99.4%, respectively in comparison with EC as the gold standard test. With Bayesian latent class modelling, EC and PCR demonstrated sensitivities of 98.7 and 99.3%, respectively, while CC showed a sensitivity of 70.3% for dete...Continue Reading

References

Jan 1, 1995·Transactions of the Royal Society of Tropical Medicine and Hygiene·A L WalshN J White
Jan 1, 1995·Transactions of the Royal Society of Tropical Medicine and Hygiene·V WuthiekanunN J White
Feb 3, 2000·Journal of Clinical Microbiology·C Winstanley, C A Hart
Dec 15, 2010·PLoS Neglected Tropical Diseases·Bart J CurrieAllen C Cheng
May 6, 2011·Transactions of the Royal Society of Tropical Medicine and Hygiene·Trinh Thanh TrungIvo Steinmetz
Nov 25, 2011·Transactions of the Royal Society of Tropical Medicine and Hygiene·Baharudin RoesnitaI-Ching Sam
Mar 8, 2013·The American Journal of Tropical Medicine and Hygiene·Allen C ChengSharon J Peacock
Apr 5, 2013·PLoS Neglected Tropical Diseases·Direk LimmathurotsakulSharon J Peacock
Apr 9, 2014·The American Journal of Tropical Medicine and Hygiene·Brea D DuvalAlex R Hoffmaster
Jan 28, 2015·Emerging Infectious Diseases·Alex R HoffmasterHenry T Walke
Feb 16, 2016·Nature Microbiology·Direk LimmathurotsakulSimon I Hay
Feb 26, 2016·Journal of Clinical Microbiology·Vichaya SuttisunhakulNarisara Chantratita
Aug 31, 2016·Nature Microbiology·Direk LimmathurotsakulSimon I Hay

❮ Previous
Next ❯

Citations

Feb 14, 2018·BMJ Case Reports·Rahul GargChiranjay Mukhopadhyay
May 29, 2020·The Indian Journal of Medical Research·Vandana Kalwaje EshwaraJordi Rello
Oct 3, 2018·Tropical Medicine and Infectious Disease·Chiranjay MukhopadhyayDavid A B Dance
Feb 18, 2021·Future Microbiology·Ian GassiepPatrick N Harris
Apr 4, 2021·Microorganisms·Kasturi SelvamIsmail Aziah

❮ Previous
Next ❯

Related Concepts