Improved detection of toxic chemicals by Photobacterium phosphoreum using modified Boss medium

Journal of Photochemistry and Photobiology. B, Biology
Sedky H A Hassan, Sang Eun Oh

Abstract

A bioluminescent bacterium Photobacterium phosphoreum has been used widely as an indicator of pollutants where the presence of toxic chemicals decreases light output. The optimum conditions for the growth and bioluminescence of P. phosphoreum KCTC 2852 were investigated using modified Boss medium and two environmental conditions (pH and temperature). Optimized conditions that supported growth of P. phosphoreum with high bioluminescence intensities were: NaCl (20 g/L), glycerol (2.5 g/L), peptone (1.0 g/L), and yeast extract (1.0 g/L). Growth and bioluminescence gradually increased as pH increased reaching a maximum at pH 7.0. The maximum temperature for growth and bioluminescence was 20 degrees C. Based on these optimum conditions for bioluminescence, a continuous culture reactor of P. phosphoreum was operated. During the continuous operation, the optimized medium maintained bioluminescence with a high relative light unit (RLU) around 13,600 for more than 120 h. When zinc (0.5 mg/L) was added to the reactor, an EC(50) was observed in 15 min. The detection limit was improved by using the modified Boss medium since the modified Boss medium contained less organic matter which minimizes the complexation and precipitation of heavy m...Continue Reading

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Citations

Nov 15, 2011·Ecotoxicology and Environmental Safety·Ying LiuLihui Huang
Apr 14, 2016·Environment International·Sedky H A HassanSang-Eun Oh
Jul 29, 2016·Ecotoxicology and Environmental Safety·Wenxin WangBin Zhou
Jul 28, 2020·International Journal of Molecular Sciences·Sherwin ReyesSapna K Deo
Feb 5, 2021·Environmental Monitoring and Assessment·Thillaichidambaram MuneeswaranChockalingam Muthiah Ramakritinan
Dec 9, 2020·Environmental Pollution·Heonseop EomSang-Eun Oh
Apr 29, 2021·Environmental Science and Pollution Research International·Ying HuangHancheng Gong
Jun 19, 2012·Environmental Science & Technology·Sedky H A HassanSang-Eun Oh
Mar 23, 2011·Environmental Science & Technology·Steven W Van GinkelSang-Eun Oh

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