Improved determination of sulpiride in plasma by ion-pair liquid chromatography with fluorescence detection

Journal of Chromatography
P NicolasO Petitjean

Abstract

A specific and sensitive high-performance liquid chromatographic method for the measurement of sulpiride in plasma is described. The internal standard used was veralipride, a structurally related substituted benzamide. A fluorescence detector with maximum excitation at 300 nm and maximum emission at 365 nm was used for quantitation. After an alkaline extraction procedure, the benzamides were separated on a 5-micron ODS column using a large organic counter ion in the mobile phase. The detector response was linear from 10 to 1000 ng/ml and the detection limit was 10 ng/ml, which is sensitive enough for pharmacokinetic studies. The suitability of the method for the analysis of biological samples was tested by studying the variation with time of plasma concentrations of sulpiride in normal human volunteers after a single therapeutic 200-mg oral dose of three different formulations of sulpiride.

Citations

Oct 29, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hea-Young Cho, Yong-Bok Lee
Aug 19, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·In Bok PaekHye Suk Lee
Nov 17, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·M C HuangM T Sheu
Mar 28, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·H TokunagaT Imamura
Oct 7, 2000·Journal of Pharmaceutical and Biomedical Analysis·O A Farghaly
Feb 1, 1991·British Journal of Clinical Pharmacology·E MagueurA M Taburet
Jun 1, 1995·The Journal of Pharmacy and Pharmacology·I NaasaniK Miyazaki
Jan 1, 1988·Ultramicroscopy·O H Griffith, G F Rempfer
Dec 12, 2012·Luminescence : the Journal of Biological and Chemical Luminescence·Muhammad Naeem KhanYoung Ho Kim
Sep 19, 2012·Luminescence : the Journal of Biological and Chemical Luminescence·Jasmin ShahSultan Shah
Jan 1, 1989·British Journal of Clinical Pharmacology·S FreestoneM R Lee
Sep 6, 2005·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Nikoletta FotakiChristos Reppas
Feb 1, 1988·British Journal of Clinical Pharmacology·T M MacDonaldM R Lee
Jun 15, 2004·Journal of Pharmaceutical and Biomedical Analysis·Su-Eon JinChong-Kook Kim
Jul 27, 2005·Journal of Molecular Modeling·Ricardo O FreireNivan B da Costa Jùnior

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.