Improved determination of urinary cortisol and cortisone, or corticosterone and 11-dehydrocorticosterone by high-performance liquid chromatography with ultraviolet absorbance detection

Journal of Chromatography. B, Biomedical Sciences and Applications
M Hay, P Mormède

Abstract

A sensitive assay was developed for the determination of low levels of free (unconjugated) glucocorticoids in human, swine (cortisol and cortisone) and rat urine (corticosterone and 11-dehydrocorticosterone), using solid-phase extraction and HPLC with UV absorbance detection (254 nm). Precise quantitation is allowed by the use of internal standards (dexamethasone for swine urine and Reichstein's substance S for rat urine). This simple method allows the use of small urine samples (less than 2 ml), and is suitable for a wide range of applications in human and animal clinical and physiological studies.

References

Mar 1, 1990·Journal of Analytical Toxicology·S J ParkJ Park
Oct 1, 1982·The Journal of Clinical Endocrinology and Metabolism·M FolleniusB Hietter
Sep 1, 1964·The Journal of Clinical Endocrinology and Metabolism·W R BEISELP H FORSHAM

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Citations

Jul 1, 2005·Meat Science·G S PlastowS C Blott
Mar 10, 2001·Physiology & Behavior·M HayP Mormède
Nov 27, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Pin Yen ShuCheng Huang Lin
Aug 1, 2007·Animal : an International Journal of Animal Bioscience·A FouryP Mormède
Mar 24, 2012·Animal : an International Journal of Animal Bioscience·A FouryP Mormède
Feb 10, 2004·Medicine and Science in Sports and Exercise·Djamila AtlaouiJean-Claude Chatard
Jun 4, 2013·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Julien AucouturierPascale Duché
Jan 20, 2007·Physiology & Behavior·Pierre MormèdeIsabelle Veissier
Sep 27, 2005·Clinical Endocrinology·Julie Vialard-MiguelJean-Benoît Corcuff
Jun 26, 2007·Biomedical Chromatography : BMC·O Al SharefS C Sharma
Oct 19, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alina PlenisJarosław Skokowski
Jul 15, 2005·Medicine and Science in Sports and Exercise·Caroline GouarnéMartine Duclos
Jan 7, 2014·Clinical Chemistry and Laboratory Medicine : CCLM·Giorgia AntonelliMario Plebani
Dec 20, 2005·Journal of Animal Science·N A GeverinkP Mormède
Jan 5, 2002·Journal of Chromatography. a·K ShimadaT Higashi
Jun 22, 2002·Journal of Chromatography. a·Patrik Appelblad, Knut Irgum
Jun 22, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·A MarwahH Lardy

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