Improved development of in vitro-derived bovine embryos by use of a nitric oxide scavenger in a cumulus-granulosa cell coculture system

Molecular Reproduction and Development
J M Lim, W Hansel

Abstract

This study was conducted to examine the hypothesis that nitric oxide (NO) affects prehatching development of bovine oocytes fertilized in vitro. In experiment 1, inseminated oocytes were cultured in a cumulus-granulosa cell (CG) coculture system to which 0.008 or 0.04 mM of sodium nitroprusside (SNP), a spontaneous NO releaser, was added at 18 or 60 hr postinsemination. Embryo development was greatly (P < 0.001) inhibited by the addition of SNP, regardless of time of addition or SNP concentration. In experiment 2, eight-cell embryos were cultured singly in a defined medium, to which 0.0016, 0.008, or 0.04 mM of SNP was added. Development to the blastocyst stage was greatly (P < 0.001) decreased after addition of SNP compared with no addition. Higher (P < 0.02) concentration of NO metabolites was found in developmentally arrested embryos than in developing embryos at 144 hr postinsemination (experiment 3). In experiment 4, blastocyst formation of oocytes cocultured with CGs was significantly (P < 0.02) increased after addition of hemoglobin (Hb, 1 microgram/ml), an NO scavenger. Prehatching development of oocytes was significantly (P < 0.05) increased after addition of Hb at different time intervals (18, 60, or 144 hr postinsemi...Continue Reading

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Citations

Mar 1, 2000·Molecular Reproduction and Development·A Jablonka-Shariff, L M Olson
Jun 1, 2001·Fertility and Sterility·H W ChenC R Tzeng
Jun 12, 2003·Fertility and Sterility·Irma Virant-KlunHelena Meden-Vrtovec
Mar 8, 2005·The Journal of Reproduction and Development·Shuntaro IkedaMasayasu Yamada
Apr 5, 2012·Reproductive Biology·In-Sul HwangJae Gyu Yoo
Jun 24, 2004·American Journal of Reproductive Immunology : AJRI·Peter J HansenRoger P Natzke
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Jan 1, 2013·Annual Review of Animal Biosciences·William Hansel
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Jul 8, 2011·Neural Computation·Emre NeftciRodney Douglas
Apr 21, 2012·Biology of Reproduction·William Hansel
Dec 15, 2007·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Liza NoordinWan Hafizah
Mar 8, 2012·Reproduction, Fertility, and Development·Robert O Gilbert
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Nov 17, 2007·Journal of Proteome Research·A Stephen GeorgiouAlireza Fazeli
May 31, 2021·Journal of Assisted Reproduction and Genetics·Megan LimKylie R Dunning

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