Improved development of somatic cell cloned mouse embryos by vitamin C and latrunculin A

PloS One
Anna MallolElena Ibáñez

Abstract

Impaired development of embryos produced by somatic cell nuclear transfer (SCNT) is mostly associated with faulty reprogramming of the somatic nucleus to a totipotent state and can be improved by treatment with epigenetic modifiers. Here we report that addition of 100 μM vitamin C (VitC) to embryo culture medium for at least 16 h post-activation significantly increases mouse blastocyst formation and, when combined with the use of latrunculin A (LatA) during micromanipulation and activation procedures, also development to term. In spite of this, no significant effects on pluripotency (OCT4 and NANOG) or nuclear reprogramming markers (H3K14 acetylation, H3K9 methylation and DNA methylation and hydroxymethylation) could be detected. The use of LatA alone significantly improved in vitro development, but not full-term development. On the other hand, the simultaneous treatment of cloned embryos with VitC and the histone deacetylase inhibitor psammaplin A (PsA), in combination with the use of LatA, resulted in cloning efficiencies equivalent to those of VitC or PsA treatments alone, and the effects on pluripotency and nuclear reprogramming markers were less evident than when only the PsA treatment was applied. These results suggest th...Continue Reading

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Citations

Feb 6, 2016·Cellular and Molecular Life Sciences : CMLS·Vladimir Camarena, Gaofeng Wang
Dec 2, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Anna MallolElena Ibanez
Feb 16, 2019·Epigenetics & Chromatin·René A M DirksHendrik Marks
Dec 15, 2019·Reproduction in Domestic Animals = Zuchthygiene·Yumei ZhangJian Hou
Apr 2, 2020·International Journal of Molecular Sciences·Chantel GouveiaMichael S Pepper
Aug 12, 2020·Cellular Reprogramming·Érika Almeida PraxedesAlexsandra Fernandes Pereira
Oct 5, 2018·Cellular and Molecular Life Sciences : CMLS·Shigeo SaitoKazunari K Yokoyama
Nov 30, 2018·Journal of Veterinary Science·Jeonghyeon Moon, Sangho Roh
Nov 11, 2019·The Journal of Biological Chemistry·Nanami KohriManabu Kawahara
Nov 21, 2017·Journal of Animal Science and Technology·A CordovaG F Mastromonaco
May 31, 2019·International Journal of Molecular Sciences·Lei ZhangZekun Guo

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Methods Mentioned

BETA
histone acetylation
acetylation
cesarian section
acethylation
immunoprecipitation

Software Mentioned

Isis
GraphPad InStat
GraphPad
ImageJ J
ImageJ

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