Improved diagnosis of pulmonary tuberculosis by detection of antibodies against multiple Mycobacterium tuberculosis antigens

Diagnostic Microbiology and Infectious Disease
Alamelu RajaRamalingam Bethunaickan

Abstract

Two secreted antigens (38 and 30 kDa) and 1 cytosolic antigen (16 kDa) were purified in our laboratory from Mycobacterium tuberculosis culture filtrate and cytosol using chromatographic/electrophoretic methods. One recombinant antigen (27 kDa, MPT51) expressed in Escherichia coli was also isolated. All the 4 antigens were tested individually for detection of serum IgG, IgA, and IgM (a total of 476 sera from 5 groups) by indirect enzyme-linked immunosorbent assay. Keeping the well-reported 38 kDa as the main candidate, the usefulness of the other antigens, which may add to the test positivity in cases not diagnosed by 38 kDa, was analyzed. The individual antigens ranged in their sensitivity from 57% to 67% (IgG). Addition of other antigen results, with that of 38 kDa, offered a sensitivity of 91% in smear- and culture-positive tuberculosis (TB), 78% in smear-negative culture-confirmed TB, and 97% specificity in normal healthy subjects. IgG antibody to multiple antigens (38, 30, and 16 kDa) may be a sensitive, specific, rapid, and cost-effective test to rule-in clinical suspicion of pulmonary TB.

References

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Jun 5, 2004·Protein Expression and Purification·B RamalingamAlamelu Raja

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Citations

Feb 4, 2011·Clinical and Vaccine Immunology : CVI·Xiu-Yun HeLin-Hu Ge
Jun 19, 2014·BMC Infectious Diseases·Xiaoyan FengHeqiu Zhang
Feb 24, 2016·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Yangjiong XiaoYing Wang
Nov 7, 2012·The Journal of Infection·Xiaoyan FengHeqiu Zhang
Jul 26, 2011·Diagnostic Microbiology and Infectious Disease·Shaoli DengMing Chen
Jul 6, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Xueqiong WuMei Dong
Jun 27, 2014·The Journal of Infection·Xiao-Lei TangXiao-Lian Zhang
Dec 3, 2014·Tuberculosis·Paramanandhan PukazhvanthenMahavir Singh
Jul 3, 2016·Clinica Chimica Acta; International Journal of Clinical Chemistry·Balaji PathakumariAlamelu Raja
Nov 4, 2009·Expert Review of Anti-infective Therapy·Faten A Al-Zamel
Dec 5, 2008·Clinical and Vaccine Immunology : CVI·Karen R SteingartSuman Laal
Sep 13, 2018·Current Medicinal Chemistry·Le Minh Tu PhanTae Jung Park
Mar 21, 2019·Drug Delivery and Translational Research·Wei WangFan Chen

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