Improved Enzyme Catalytic Characteristics upon Glutaraldehyde Cross-Linking of Alginate Entrapped Xylanase Isolated from Aspergillus flavus MTCC 9390

Enzyme Research
Bharat BhushanVeena Jain

Abstract

Purified fungal xylanase was entrapped in alginate beads. Its further cross-linking using glutaraldehyde resulted in large enzyme aggregates which may function as both a catalyst and a support material for numerous substrate molecules. Enzyme cross-linking presented a negative impact on enzyme leaching during repeated washings and recovery of enzyme activity was substantial after twelve cycles of usage. The entrapment followed by cross-linking doubled the total bound activity and also greatly improved the enzyme stability at extreme chemical environment. The wide pH stability, better thermo- and storage stability, lowered K m value, and protection from some metal ions are salient achievements of present immobilization. The study shows the efficacy, durability, and sustainability of immobilized catalytic system which could be efficiently used for various juice processing operations.

References

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Citations

Dec 20, 2016·Journal of Food Science·Chieh-Ting ChenKuan-Chen Cheng
Sep 29, 2018·Preparative Biochemistry & Biotechnology·Adegoke Isiaka Adetunji, Ademola Olufolahan Olaniran
Dec 14, 2018·International Journal of Molecular Sciences·Cristina CattòFrancesca Cappitelli
Nov 28, 2018·Applied Biochemistry and Biotechnology·Jorge GracidaAldo Amaro-Reyes
Nov 1, 2020·Applied Microbiology and Biotechnology·Xiaolei GuoJianzhong Huang
Jan 26, 2021·International Journal of Biological Macromolecules·Agne SavickaiteRenata Gudiukaite
Feb 12, 2020·Enzyme and Microbial Technology·Daoyuan YangJosé I Reyes-De-Corcuera

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Methods Mentioned

BETA
molecular exclusion chromatography

Related Concepts

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Aspergillosis (ASM)

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Aspergillosis

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.