Improved ethanol and reduced xylitol production from glucose and xylose mixtures by the mutant strain of Candida shehatae ATCC 22984

Applied Biochemistry and Biotechnology
Yuan LiKen Tokuyasu

Abstract

A mutant Cs3512, which showed better fermentation of xylose and the mixtures of xylose and glucose, was obtained through mutation of Candida shehatae ATCC 22984 and screening with a medium containing antimycin A and TTC (2,3,5-triphenyltetrazolium chloride). Cs3512 produced 44.4 g/l of ethanol from 121.3 g/l of xylose, which was 13% higher than that by ATCC 22984. At the same time, xylitol production was reduced by 38% to 10.2 g/l from 16.3 g/l by ATCC 22984. Cs3512 also showed 8% increase in ethanol yield from 0.39 to 0.42 g/g comparing to ATCC 22984 when fermenting the sugar mixture composed of 52.9 g/l glucose and 21.2 g/l xylose. When Cs3512 was used in the simultaneous saccharification and fermentation of lime pretreated rice straw via CaCCO (calcium capturing by carbonation) process, it produced ethanol at 77% of the theoretical yield. The results imply that Cs3512 is a potential non-recombinant yeast strain for ethanol production from lignocellulosic biomass.

References

Oct 10, 1997·Enzyme and Microbial Technology·S SánchezF Camacho
Nov 21, 1998·Journal of Biotechnology·M DrögeW Selbitschka
Sep 13, 2002·Applied Microbiology and Biotechnology·M Galbe, G Zacchi
Apr 12, 2003·FEMS Yeast Research·Alexandra VeigaMaria C Loureiro-Dias
Dec 5, 1992·Biotechnology and Bioengineering·J R KastnerR S Roberts
Dec 30, 2008·Journal of Industrial Microbiology & Biotechnology·Yuan LiNorio Sugiura
May 8, 2009·Bioscience, Biotechnology, and Biochemistry·Jeung-yil ParkKen Tokuyasu
May 20, 2009·Biotechnology for Biofuels·Luen-Luen LiDaniel van der Lelie
Jun 9, 2009·Current Opinion in Biotechnology·Antoine MargeotFrédéric Monot
Jan 13, 2010·Journal of Industrial Microbiology & Biotechnology·Romain FromangerX Cameleyre

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Citations

Jan 9, 2013·Applied Biochemistry and Biotechnology·Jinjing WangQi Li
Nov 19, 2014·Journal of Industrial Microbiology & Biotechnology·Nicole K HarnerHung Lee
Dec 15, 2020·FEMS Microbiology Reviews·Justyna Ruchala, Andriy A Sibirny

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