PMID: 9830094Nov 27, 1998Paper

Improved ethanol production by glycerol-3-phosphate dehydrogenase mutants of Saccharomyces cerevisiae

Applied Microbiology and Biotechnology
H ValadiL Gustafsson

Abstract

The anaerobic performance of gpd1 delta and gpd2 delta mutants of Saccharomyces cerevisiae was characterized and compared to that of a wild-type strain under well-controlled conditions by using a high-performance bioreactor. There was a 40% reduction in glycerol level in the gpd2 delta mutant compared to the wild-type. Also the gpd1 delta mutant showed a slight decrease in glycerol formation but to a much lesser degree. As a consequence, ethanol formation in the gpd2 delta mutant was elevated by 13%. In terms of growth, the gpd1 delta mutant and the wild-type were indistinguishable. The gpd2 delta mutant, on the other hand, displayed an extended lag phase as well as a reduced growth rate under the exponential phase. Even though glycerol-3-phosphate dehydrogenase 2 (GPD2) is the important enzyme under anaerobic conditions it can, at least in part, be substituted by GPD1. This was indicated by the higher expression level of GPD1 in the gpd2 delta mutant compared to the wild type. These results also show that the cells are able to cope and maintain redox balance under anaerobic conditions even if glycerol formation is substantially reduced, as observed in the gpd2 delta mutant. One obvious way of solving the redox problem would be...Continue Reading

Citations

Oct 6, 2006·Applied Microbiology and Biotechnology·Qing-Xue KongXun Chen
May 17, 2007·Applied Microbiology and Biotechnology·Qing-Xue KongXun Chen
Nov 20, 2008·Applied Microbiology and Biotechnology·Zhong-peng GuoGui-Yang Shi
Sep 22, 2001·Journal of Microbiological Methods·H ValadiL Gustafsson
Jul 5, 2011·Applied and Environmental Microbiology·Georg HubmannElke Nevoigt
Mar 8, 2000·Microbiology and Molecular Biology Reviews : MMBR·S OstergaardJ Nielsen
May 21, 2011·BMC Systems Biology·Felipe A VargasEduardo Agosin
Jul 26, 2002·Molecular Microbiology·Uros PetrovicAna Plemenitas
Sep 30, 2010·Journal of Industrial Microbiology & Biotechnology·Annamaria MericoConcetta Compagno
Oct 7, 2008·Applied Microbiology and Biotechnology·Roberto Pérez-TorradoEmilia Matallana
Oct 11, 2003·Biotechnology Advances·Z X WangB A Prior
Jan 30, 2007·Letters in Applied Microbiology·A ZhangX Chen
Apr 25, 2013·Current Opinion in Biotechnology·Jens NielsenJack Pronk
Apr 19, 2003·FEMS Yeast Research·Roeland CostenobleGunnar Lidén
Oct 22, 2014·Molecular BioSystems·C AiroldiP Coccetti
Dec 13, 2000·Yeast·R CostenobleC J Franzén
Oct 27, 2018·BioMed Research International·Maria Bolivar-TelleriaPatricia M B Fernandes

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