Abstract
Genome shuffling by recursive protoplast fusion between Saccharomyces cerevisiae and Pichia stipitis also known as Scheffersomyces stipitis resulted in a promising yeast hybrid strain with superior qualities than those of the parental strains in enhancing biofuel production. Our study focused on the substrate utilization, ethanol fermentation, and ethanol tolerance of the hybrids and the parental strains. The parental strain S. cerevisiae is limited to utilize only hexose sugars, and this leads to decrease in the ethanol yield when they are subjected to ethanol production from lignocellulosic biomass which is rich in pentose sugars. To overcome this limitation, we constructed a hybrid yeast strain through genome shuffling which can assimilate all the sugars present in the fermentation medium. After two rounds of recursive protoplast fusion, there was a higher increase in substrate utilization by hybrid SP2-18 compared to parental strain S. cerevisiae. SP2-18 was able to consume 34% of xylose sugar present in the fermentation medium, whereas S. cerevisiae was not able to utilize xylose. Further, the hybrid strain SP2-18 was able to reach an ethanol productivity of 1.03 g L-1 h-1, ethanol yield 0.447 g/g, and ethanol concentratio...Continue Reading
References
Oct 11, 1991·Nucleic Acids Research·J Welsh, M McClelland
Nov 25, 1990·Nucleic Acids Research·J G WilliamsS V Tingey
Jan 1, 1986·Current Genetics·B P Curran, B L Carter
Jan 26, 1996·The Journal of Biological Chemistry·Q LiuM Forgac
Aug 24, 1999·Canadian Journal of Microbiology·T M Swan, K Watson
Feb 8, 2002·Nature·Ying-Xin ZhangStephen B del Cardayré
Jun 29, 2002·Nature Biotechnology·Gregory Stephanopoulos
Jun 29, 2002·Nature Biotechnology·Ranjan PatnaikStephen del Cardayré
Apr 23, 2004·BMC Biotechnology·Susanna HarjuKenneth R Peterson
Sep 9, 2006·Microbiological Research·T LebeauG-A Junter
Dec 26, 2007·Bioresource Technology·Oscar J Sánchez, Carlos A Cardona
May 17, 2008·Bioresource Technology·Rojan P JohnK Madhavan Nampoothiri
Aug 30, 2008·Applied Biochemistry and Biotechnology·Fei YanXiushan Yang
Oct 22, 2008·Journal of Bioscience and Bioengineering·Akinori Matsushika, Shigeki Sawayama
May 26, 2009·Biotechnology Advances·Jixian GongXueming Zhao
Oct 27, 2009·Applied and Environmental Microbiology·Burkhard OtteStefan Jennewein
Dec 19, 2009·Bioresource Technology·Rajeev K SukumaranAshok Pandey
Jun 18, 2010·Journal of Biotechnology·Ying ZhangZong-Wan Mao
Aug 31, 2010·Biotechnology Letters·Zong-wen PangRi-bo Huang
Jan 25, 2012·Journal of Industrial Microbiology & Biotechnology·Ge JingpingPing Wenxiang
May 16, 2012·Journal of Food Science·Zaki A El-FikyAhmed M Emam
Jul 20, 2012·Biotechnology for Biofuels·Wei Zhang, Anli Geng
Jan 28, 2014·PloS One·Liwei ChenWei Ning Chen
Jun 17, 2014·Microbiological Research·Chizuru YamaokaTomoko Kubo
Dec 17, 2015·Ambio·Eva-Mari Aro
Citations
Nov 14, 2019·Current Microbiology·Liang ChenYuan-Sen Hu
Sep 25, 2020·World Journal of Microbiology & Biotechnology·Liang ChenKe Bian
Feb 4, 2021·Microorganisms·Thomas SchalckJan Michiels
Jun 20, 2020·Biotechnology Advances·Jinyu CuiWeiwen Zhang
Mar 27, 2021·Nature Reviews. Microbiology·Jan SteenselsKevin J Verstrepen
Aug 5, 2021·Microbiology Spectrum·Xiaoling ChenDong Chen