Improved ethanol productivity and ethanol tolerance through genome shuffling of Saccharomyces cerevisiae and Pichia stipitis

International Microbiology : the Official Journal of the Spanish Society for Microbiology
Karuna Devi JettiSai Kishore Nammi

Abstract

Genome shuffling by recursive protoplast fusion between Saccharomyces cerevisiae and Pichia stipitis also known as Scheffersomyces stipitis resulted in a promising yeast hybrid strain with superior qualities than those of the parental strains in enhancing biofuel production. Our study focused on the substrate utilization, ethanol fermentation, and ethanol tolerance of the hybrids and the parental strains. The parental strain S. cerevisiae is limited to utilize only hexose sugars, and this leads to decrease in the ethanol yield when they are subjected to ethanol production from lignocellulosic biomass which is rich in pentose sugars. To overcome this limitation, we constructed a hybrid yeast strain through genome shuffling which can assimilate all the sugars present in the fermentation medium. After two rounds of recursive protoplast fusion, there was a higher increase in substrate utilization by hybrid SP2-18 compared to parental strain S. cerevisiae. SP2-18 was able to consume 34% of xylose sugar present in the fermentation medium, whereas S. cerevisiae was not able to utilize xylose. Further, the hybrid strain SP2-18 was able to reach an ethanol productivity of 1.03 g L-1 h-1, ethanol yield 0.447 g/g, and ethanol concentratio...Continue Reading

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Citations

Sep 25, 2020·World Journal of Microbiology & Biotechnology·Liang ChenKe Bian
Jun 20, 2020·Biotechnology Advances·Jinyu CuiWeiwen Zhang
Mar 27, 2021·Nature Reviews. Microbiology·Jan SteenselsKevin J Verstrepen

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Methods Mentioned

BETA
electrophoresis
five hybrid
five hybrids

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