Improved evaporative light scattering detection for carbohydrate analysis

Food Chemistry
Luis Condezo-HoyosPilar Rupérez

Abstract

Optimization and validation of evaporative light scattering detector (ELSD), aided by response surface methodology (RSM), has been developed for the liquid chromatography analysis of a wide molecular weight (MW) range of carbohydrates, including polysaccharides and oligosaccharides. Optimal experimental parameters for the ELSD detection were: 88.8°C evaporator temperature, 77.9°C nebulizer temperature and 1.1 standard litres per minute nitrogen flow rate. Optimal ELSD detection, used together with high performance size exclusion chromatography (HPSEC) of carbohydrates, gave a linear range from 250 to 1000 mg L(-1) (R(2)>0.998), with limits of detection and quantitation of 4.83-11.67 and 16.11-38.91 mg L(-1), respectively. Relative standard deviation was lower than 1.8% for intra-day and inter-day repeatability for apple pectin, inulin, verbascose, stachyose and raffinose. Recovery ranged from 103.7% to 118.3% for fructo-oligosaccharides, α-galacto-oligosaccharides and disaccharides. Optimized and validated ELSD detection is proposed for the analysis of high- to low-MW carbohydrates with high sensitivity, precision and accuracy.

References

Feb 13, 2007·Journal of Pharmaceutical and Biomedical Analysis·Joseph Rashan, Raymond Chen
Aug 9, 2007·Analytica Chimica Acta·S L C FerreiraW N L dos Santos
Aug 31, 2010·Talanta·Eva Gómez-OrdóñezPilar Rupérez

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Citations

Mar 3, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lidong CaoQiliang Huang
Aug 9, 2020·Foods·Rocío De la Peña-ArmadaInmaculada Mateos-Aparicio

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