Improved fluorometric enzymatic sorbitol assay in human blood

Clinica Chimica Acta; International Journal of Clinical Chemistry
R ShinoharaI Ishiguro

Abstract

Samples for use in the fluorometric enzymatic assay of sorbitol in erythrocytes are normally prepared using HClO4 and K2CO3. We have replaced these reagents with NaOH and ZnSO4. Human whole blood, erythrocyte and plasma samples prepared with NaOH and ZnSO4 are colorless and clear, while erythrocyte samples prepared with HClO4 and K2CO3 are a pale yellow-brown color. The sorbitol dehydrogenase reaction in the supernatant of the mixture of NaOH and ZnSO4 is inhibited, but ethylenediaminetetraacetate completely eliminates this effect. The sorbitol assay in erythrocytes prepared with NaOH and ZnSO4 shows higher sensitivity and reproducibility than did that with HClO4 and K2CO3. Recovery of sorbitol added to erythrocytes is similar in both assay methods. Concentrations of whole blood and erythrocyte sorbitol assayed by the present method are significantly higher in diabetics than in normals. Poorly controlled diabetics had higher whole blood and erythrocyte sorbitol than well-controlled diabetics. Whole blood sorbitol concentrations differed more between diabetic and normal subjects than did erythrocyte sorbitol concentrations.

References

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Citations

Oct 30, 2003·Journal of Diabetes and Its Complications·Itsuko NakanoAkio Nagasaka
Jan 8, 2011·Biological & Pharmaceutical Bulletin·Jin Kyu KimSoon Sung Lim
Oct 24, 2012·Journal of Biomedicine & Biotechnology·Hong Mei LiSoon Sung Lim
Jul 17, 2007·Research in Veterinary Science·S ComazziP Sartorelli
Apr 16, 2013·BioMed Research International·Tae Hyeon KimSoon Sung Lim
Sep 27, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hong Mei LiSoon Sung Lim
Aug 19, 2017·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Tomasz RusakMarian Tomasiak
Dec 5, 1998·Biochimica Et Biophysica Acta·R ShinoharaM Itoh

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