Improved gene expression pattern using Epstein-Barr virus (EBV)-based plasmid and cationic emulsion

Biomaterials
Kyoung Ah MinChong-Kook Kim

Abstract

To improve transgene expression of a non-viral gene delivery system, an Epstein-Barr virus (EBV)-based plasmid and cationic emulsion complex was prepared and evaluated. Cationic emulsion was formulated with castor oil, 3-N-(N',N'-dimethylaminoethane)-carbamoyl cholesterol (DC-Chol) and other co-emulsifiers. An EBV-based plasmid containing the two EBV components, origin of replication (oriP) and EBV nuclear antigen 1 (EBNA-1), was constructed. The physical characteristics of the emulsion and the emulsion/DNA complex were determined. After cells were transfected with cationic emulsion/EBV-based plasmid complex, transfection efficiency and expression pattern were evaluated using green fluorescent protein (GFP) as a reporter. The average particle size and zeta potential of the emulsion itself were 96 nm and + 17 mV, respectively. The emulsion showed stable size distribution up to at least one month. With an increase of emulsion to DNA ratio, zeta-potential increased from negative to positive and the particle size decreased to 200-300 nm. The complex was stable against DNase I digestion and showed comparable transfection efficiency with Lipofectin for several tested cell lines. An enhanced and prolonged gene expression was achieved ...Continue Reading

References

Aug 11, 1995·The Journal of Biological Chemistry·J ZabnerM J Welsh
May 2, 1996·The New England Journal of Medicine·J M Wilson
Oct 1, 1996·Histopathology·I Anagnostopoulos, M Hummel
May 21, 1998·Critical Reviews in Therapeutic Drug Carrier Systems·A P Rolland
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·G J Nabel
Feb 4, 1999·Gene Therapy·O ZelphatiP L Felgner
Apr 13, 2000·Journal of Drug Targeting·J M Benns, S W Kim
Aug 11, 1997·Advanced Drug Delivery Reviews·J Zabner
Jun 3, 2004·Biomaterials·Woo-Jeong ChoiChong-Kook Kim

❮ Previous
Next ❯

Citations

Oct 19, 2005·Gene Therapy·M TuomelaK Krohn
Nov 19, 2008·Journal of Drug Targeting·Hyun-Suk KangJeong-Sook Park

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.