Improved genetic resolution for linkage mapping of resistance to potato wart in monoparental dihaploids with potential diagnostic value in tetraploid potato varieties

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Annette M BartkiewiczThomas Debener

Abstract

We achieved improved mapping resolution of the major wart resistance locus Xla-TNL containing also Sen1 in a dihaploid population using SNP data and developed additional markers with diagnostic value in tetraploid varieties. We analyzed a segregating monoparental dihaploid potato population comprising 215 genotypes derived from a tetraploid variety that is highly resistant to Synchytrium endobioticum pathotypes 18 and 6. The clear bimodal segregation for both pathotypes indicated that a major dominant resistance factor in a simplex allele configuration was present in the tetraploid donor genotype. Compared to that in previous analyses of the same tetraploid donor in conventional crosses with susceptible tetraploid genotypes, a segregation pattern with a reduced genetic complexity of resistance in dihaploids was observed here. Using the 12.8 k SolCAP SNP array, we mapped a resistance locus to the Xla-TNL region containing also Sen1 on potato chromosome 11. The improved mapping resolution provided by the monoparental dihaploids allowed for the localization of the genes responsible for the resistance to both pathotypes in an interval spanning less than 800 kbp on the reference genome. Furthermore, we identified eight molecular mar...Continue Reading

References

Feb 5, 2000·Nature Biotechnology·M Schuelke
Jul 27, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Ye-Su SongAndrea Schwarzfischer
Nov 2, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Bart BrugmansHerman J van Eck
Mar 16, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·C GebhardtJ P T Valkonen
Feb 1, 1993·Genome Génome / Conseil National De Recherches Canada·O PinedaS D Tanksley
Aug 2, 2008·International Journal of Plant Genomics·Luciano Carlos da MaiaAntonio Costa de Oliveira
May 19, 2011·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Erin BakkerAska Goverse
May 5, 2012·PloS One·Kimberly J FelcherDavid S Douches
Jul 1, 1995·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·J M JacobsW J Stiekema
Jul 1, 1989·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·C GebhardtF Salamini
Feb 8, 2014·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Jude Ejikeme ObidiegwuChristiane Gebhardt
Jan 1, 2009·Language Learning and Development : the Official Journal of the Society for Language Development·Daniel J WeissAaron D Mitchel
Jun 6, 2018·Frontiers in Plant Science·Annette M BartkiewiczThomas Debener

❮ Previous
Next ❯

Citations

Feb 12, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Charlotte ProdhommeHerman J van Eck
Sep 13, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Charlotte ProdhommeJack H Vossen
Jan 15, 2022·Molecular Plant Pathology·Bart T L H van de VossenbergTheo A J van der Lee

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
genotyping
PCR
Infrared Imaging
electrophoresis
RNA-Seq

Software Mentioned

JoinMap
SynTest
Spud DB Genome Browser PGSC
SSRLocatorI
R

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.