Improved high-performance liquid chromatography (HPLC) method for qualitative and quantitative analysis of allantoin in Zea mays

Journal of Agricultural and Food Chemistry
Ghasem HaghiAlireza Safaei

Abstract

A high-performance liquid chromatography (HPLC) method for the qualitative and quantitative analysis of allantoin in silk and seed of Zea mays has been developed. Allantoin separation in crude extract was achieved using a C 18 column and phosphate buffer solution (pH 3.0) as a mobile phase at ambient temperature at a flow rate of 1.0 mL/min and detected at 210 nm. The results showed that the amount of allantoin in samples was between 14 and 271 mg/100 g of dry plant material. A comprehensive validation of the method including sensitivity, linearity, repeatability, and recovery was conducted. The calibration curve was linear over the range of 0.2-200 microg/mL with a correlation coefficient of r2>0.999. Limit of detection (LOD, S/N=3) and limit of quantification (LOQ) values of the allantoin were 0.05 and 0.2 microg/mL (1.0 and 4.0 ng) respectively. The relative standard deviation (RSD) value of the repeatability was reported within 1.2%. The average recovery of allantoin added to samples was 100.6% with RSD of 1.5%.

References

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Citations

Mar 7, 2012·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Raquel Rennó BragaSheila Garcia
Mar 10, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Takahiro DoiShozo Iwagami
Jun 30, 2018·The New Phytologist·Xue-Fang YangChui-Hua Kong
Sep 6, 2008·Phytochemical Analysis : PCA
Nov 5, 2019·Acta Pharmaceutica : a Quarterly Journal of Croatian Pharmaceutical Society and Slovenian Pharmaceutical Society, Dealing with All Branches of Pharmacy and Allied Sciences·Katarzyna KimelMirosława Krauze-Baranowska

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