Apr 28, 2004

Improved immunogenicity of novel baculovirus-derived Theileria parva p67 subunit antigens

Veterinary Parasitology
Stephen A KabaMonique M van Oers

Abstract

East Coast fever (ECF) in cattle is caused by the tick-borne protozoan parasite Theileria parva. The major sporozoite surface antigen of T. parva (p67) is an important candidate for inclusion in a subunit vaccine. Recently, we reported the expression and production of different parts of p67 as fusions to either GFP or to the baculovirus GP64 envelope glycoprotein in insect cells, which resulted in stable proteins recognized by a monoclonal specific for native p67. The immunogenicity of these fusion proteins was examined in out-bred mice and cattle. In mice, the full length p67 molecule without its signal peptide and transmembrane region, but fused to GFP (GFP:p67deltaSS) was the best immunogen followed by the C-terminus of p67 fused to GP64 (GP64:p67C). These two immunogens also provoked a high level of sero-conversion in cattle when formulated in a water-in-oil or saponin-derived adjuvant with only 100 microg of protein and a single booster. The vaccine-elicited antibodies efficiently inhibited the infectivity of T. parva sporozoites in in vitro neutralization assays. This study demonstrated that these new baculovirus-derived p67 vaccines were highly immunogenic, and that in combination with a suitable adjuvant, they have a cl...Continue Reading

Mentioned in this Paper

Saponin
Neutralising Antibodies Analysis
Oryctes Viruses
Monoclonal Antibodies
Ethanol
Gene Products, env
Serology (Antibodies and Most Antigens Except Blood Bank and Infectious Agents)
Variolation
Glycerin
Ethanol Measurement

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