Jan 1, 1989

Improved immunohistochemical detection of prostatic acid phosphatase by a monoclonal antibody

The Prostate
K W LamS C Ziesmer


A monoclonal antibody with high affinity to acid phosphatase isoenzyme 2 (Ab-AcP2) was selected to examine its binding to different normal and tumor tissues using the indirect immunohistochemical method. Both mature prostatic epithelial cells in the prostate and the highly dedifferentiated prostatic cancer cells in the bone marrow showed strong binding to the antibody. Among nonprostatic tissues, only bone marrow, breast, and kidney showed trace staining in some specimens. The specificity of Ab-AcP2 was much better than that of the polyclonal antibody to acid phospatase previously reported. When the antibody to the prostate-specific antigen (Ab-PSA) was used, weak background staining was often encountered, and weak to moderate stains were seen in the prostatic stroma, bone marrow, lung, skin, and melanoma.

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Mentioned in this Paper

Monoclonal Antibodies
Prostatic Neoplasms
Acid Phosphatase
KLK3 gene
Squamous Transitional Epithelial Cell Count
Bone Marrow
Polyclonal antibody

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